Histochemical and immunohistochemical evidence for hepatic zone 3 distribution of alcohol dehydrogenase in rats

Shinzo Kato, Hiromasa Ishii, Sadakazu Aiso, Shuji Yamashita, Daisuke Ito, Masaharu Tsuchiya

研究成果: Article査読

20 被引用数 (Scopus)

抄録

The distribution of alcohol dehydrogenase in the hepatic acinus was examined by both histochemical and immunohistochemical approaches. The immunohistochemical method using anti–alcohol dehydrogenase antibody indicated zone 3 predominance of this enzyme in the hepatic acinus, whereas a conventional histochemical method showed slight zone 1 predominance. However, when the histochemical technique was improved by using 2% glutaraldehyde instead of formalin for fixation and by adding phenazine methosulfate (0.33 mmol/L) to the staining incubation mixture, this method also supported zone 3 predominance of alcohol dehydrogenase. Evidence for zone 3 distribution of alcohol dehydrogenase may be of value in elucidating the mechanism of zone 3 liver damage by alcohol. (HEPATOLOGY 1990;12:66–69).

本文言語English
ページ(範囲)66-69
ページ数4
ジャーナルHepatology
12
1
DOI
出版ステータスPublished - 1990 7
外部発表はい

ASJC Scopus subject areas

  • 肝臓学

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