Immunodeficient NOD-scid IL-2Rγnull mice do not display T and B cell leakiness

Ikumi Katano, Ryoji ITO, Tomoo Eto, Sadakazu Aiso, Mamoru Ito

研究成果: Comment/debate査読

16 被引用数 (Scopus)

抄録

NOD/Shi-scid IL-2Rγnull (NOG) mice established by introducing the IL-2Rγnull gene of IL-2Rγ KO mice into NOD/Shi-scid mice by backcross-mating show a high xenograft engraftment level and are therefore well suited as a humanized mouse model. SCID mice bearing the Prkdcscid gene show a high incidence of thymic lymphoma and a leaky phenomenon in which a few clonal T and B cells develop in aged mice. In the present study, NOG mice were assessed for the presence of a leaky phenomenon such as the one observed in C.B-17-scid and NOD-scid mice. Serum immunoglobulin analysis did not detect IgG or IgM in NOG mice, unlike the findings in C.B-17-scid and NOD-scid mice. Flow cytometry analysis revealed the absence of T and B cells in the peripheral blood and spleens of NOG mice. These results reflect the suppression of the leaky phenomenon in NOG mice through the inactivation of the IL-2Rγ gene, which is commonly expressed in T and B cell growth factor receptors to IL-2, IL-4 and IL-7.

本文言語English
ページ(範囲)181-186
ページ数6
ジャーナルExperimental Animals
60
2
DOI
出版ステータスPublished - 2011
外部発表はい

ASJC Scopus subject areas

  • 動物科学および動物学
  • 生化学、遺伝学、分子生物学(全般)
  • 獣医学(全般)

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