Implanted cannula-mediated repetitive administration of Aβ25-35 into the mouse cerebral ventricle effectively impairs spatial working memory

Marina Yamada, Tomohiro Chiba, Jumpei Sasabe, Mikiro Nawa, Hirohisa Tajima, Takako Niikura, Kenzo Terashita, Sadakazu Aiso, Yoshiko Kita, Masaaki Matsuoka, Ikuo Nishimoto

研究成果: Article査読

54 被引用数 (Scopus)

抄録

Amyloid β (Aβ) is closely related to the onset of Alzheimer's disease (AD). To construct AD animal models, a bolus administration of a large dose of toxic Aβ into the cerebral ventricles of rodents has been performed in earlier studies. In parallel, a continuous infusion system via an osmotic pump into the cerebral ventricle has been developed to make a rat AD model. In this study, we developed a mouse AD model by repetitive administration of Aβ25-35 via a cannula implanted into the cerebral ventricle. Using this administration system, we reproducibly constructed a mouse with impaired spatial working memory. In accordance with the occurrence of the abnormal mouse behavior, we found that the number of choline acetyltransferase (ChAT)-positive neurons was reduced in paraventricular regions of brains of Aβ25-35- administered mice in a dose-dependent manner. Considering that the repetitive administration of a small dose of toxic Aβ via an implanted cannula leads to a brain status more resembling that of the AD patients than a bolus injection of a large dose of Aβ, and therapeutic as well as toxic agents are able to be repeatedly and reliably administered via an implanted cannula, we concluded that the implanted cannula-bearing AD mouse model is useful for development of new AD therapy.

本文言語English
ページ(範囲)139-146
ページ数8
ジャーナルBehavioural Brain Research
164
2
DOI
出版ステータスPublished - 2005 11月 7
外部発表はい

ASJC Scopus subject areas

  • 行動神経科学

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