In vitro antioxidative activity of ( - )-epicatechin glucuronide metabolites present in human and rat plasma

Midori Natsume, Naomi Osakabe, Akiko Yasuda, Seigo Baba, Takashi Tokunaga, Kazuo Kondo, Toshihiko Osawa, Junji Terao

研究成果: Article査読

52 被引用数 (Scopus)


Recently we identified four conjugated glucuronide metabolites of epicatechin, (-)-epicatechin-3′-O-glucuronide (E3′G), 4′-O-methyl-(-)-epicatechin-3′-O-glucuronide (4′ ME3′G), (-)-epicatechin-7-O-glucuronide (E7G) and 3′-O-methyl-(-)-epicatechin-7-O-glucuronide (3′ME7G) from plasma and urine. E3′G and 4′ME3′G were isolated from human urine, while E7G and 3′ME7G were isolated from rats that had received oral administration of (-)-epicatechin (Natsume et al. (2003), Free Radic. Biol. Med. 34, 840-849). It has been suggested that these metabolites possess considerable in vivo activity, and therefore we carried out a study to compare the antioxidant activities of the metabolites with that of the parent compound. This was achieved by measuring superoxide scavenging activity, reduction of plasma TBARS production and reduced susceptibility of low-density-lipoprotein (LDL) to oxidation. (-)-Epicatechin was found to have more potent antioxidant activity than the conjugated glucuronide metabolites. Both (- -epicatechin and E7G had marked antioxidative properties with respect to superoxide radical scavenging activity, plasma oxidation induced by 2,2′-azobis-(2-aminopropane) dihydrochloride (AAPH) and LDL oxidation induced by copper ions or 2,2′-azobis(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN). In contrast, the other metabolites had light antioxidative activities over the range of physiological concentrations found in plasma.

ジャーナルFree Radical Research
出版ステータスPublished - 2004 12月 1

ASJC Scopus subject areas

  • 生化学


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