In vivo assessment of bone marrow cell implantation in reconstructive surgery using microcirculatory experimental models

S. Ichioka, Masahiro Shibata, S. Kudo, N. Sekiya, T. Nakatsuka

研究成果: Conference contribution

抄録

Regenerative medicine using manipulated stem cells or progenitors has become a center of attraction as advancing and epoch-making medical science. One of the candidates for available progenitors includes bone marrow cells (BMCs). Using our original microcirculatory experimental models, we investigated the effects of implantation of BMCs to access its possibility of clinical application in reconstructive surgery. We used two experimental models; the mouse symmetrical flap model and the visualized angiogenesis model. The former provides reliable method for the study of ischemia-reperfusion injury. The latter allows analysis of microcirculation during wound healing angiogenesis under an intravital microscope. BMCs from the mouse femur and tibia were prepared so as to endothelial progenitor cell (EPC)-enriched fraction was isolated. Prepared BMCs were applied to the two experimental models. Local implantation of BMCs significantly improved flap survival area in the symmetrical flap model. BMC administration positively influenced microcirculatory responses in angiogenic process in the wound healing model. The present study indicates that ischemia-reperfusion injury and wound healing angiogenesis may be successfully treated by BMC implantation.

元の言語English
ホスト出版物のタイトルAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ページ686
ページ数1
1
出版物ステータスPublished - 2002
外部発表Yes
イベントProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
継続期間: 2002 10 232002 10 26

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
United States
Houston, TX
期間02/10/2302/10/26

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Surgery
Bone
Cells
Microcirculation
Endothelial cells
Stem cells
Microscopes

ASJC Scopus subject areas

  • Bioengineering

これを引用

Ichioka, S., Shibata, M., Kudo, S., Sekiya, N., & Nakatsuka, T. (2002). In vivo assessment of bone marrow cell implantation in reconstructive surgery using microcirculatory experimental models. : Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (巻 1, pp. 686)

In vivo assessment of bone marrow cell implantation in reconstructive surgery using microcirculatory experimental models. / Ichioka, S.; Shibata, Masahiro; Kudo, S.; Sekiya, N.; Nakatsuka, T.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 巻 1 2002. p. 686.

研究成果: Conference contribution

Ichioka, S, Shibata, M, Kudo, S, Sekiya, N & Nakatsuka, T 2002, In vivo assessment of bone marrow cell implantation in reconstructive surgery using microcirculatory experimental models. : Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 巻. 1, pp. 686, Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS), Houston, TX, United States, 02/10/23.
Ichioka S, Shibata M, Kudo S, Sekiya N, Nakatsuka T. In vivo assessment of bone marrow cell implantation in reconstructive surgery using microcirculatory experimental models. : Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 巻 1. 2002. p. 686
Ichioka, S. ; Shibata, Masahiro ; Kudo, S. ; Sekiya, N. ; Nakatsuka, T. / In vivo assessment of bone marrow cell implantation in reconstructive surgery using microcirculatory experimental models. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. 巻 1 2002. pp. 686
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