In vivo cloning of arylsulfatase-tyramine oxidase genes in rec strains of klebsiella aerogenes

Yoshikatsu Murooka, Masahide Oka, Mitsuo Yamashita, Masanori Sugiyama, Tokuya Harada

研究成果: Article査読

5 被引用数 (Scopus)

抄録

A general, genetic technique for in vivo cloning of bacterial genes is presented. We previously introduced the Mu phage into various genera of bacteria including Klebsiella aerogenes with RP4:: Mu. Using these strains carrying RP4:: Mu cts and thermo-inducible Mu prophage in the chromosome, we cloned in vivo the arylsulfatase (ats) and tyramine oxidase (tyn) genes by partial thermo-induction. The donor strains carrying the recombinant plasmids were conjugated with K. aerogenes rec strains, which were isolated as UV-sensitive mutants. The resultant recombinant plasmids, pATl and pAT2, were purified and used for the transformation of mutant strains deficient in the ats and tyn genes. The ats-tyn genes seemed to be transposed into the RP4::Mu cts plasmid together with other chromosomal DNA fragments. This in vivo cloning method is applicable to a wide variety of gram-negative bacteria.

本文言語English
ページ(範囲)1807-1815
ページ数9
ジャーナルAgricultural and Biological Chemistry
47
8
DOI
出版ステータスPublished - 1983 8
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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