Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells

H. J. Cho, K. P. Choi, Mitsuo Yamashita, H. Morikawa, Y. Murooka

研究成果: Article

28 引用 (Scopus)

抄録

We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line.

元の言語English
ページ(範囲)133-138
ページ数6
ジャーナルApplied Microbiology and Biotechnology
44
発行部数1-2
DOI
出版物ステータスPublished - 1995 12
外部発表Yes

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Cholesterol Oxidase
Tobacco
Cholesterol
Genes
Proteins
Oxidoreductases
Viruses
Pneumatics
Plasmids
Cells

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology
  • Bioengineering
  • Biotechnology

これを引用

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AU - Murooka, Y.

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