Isolation and characterization of a mouse SRY-related cDNA, mSox7

Koji Taniguchi; Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Susumu Kido; Sadakazu Aiso

doi:10.1016/S0167-4781(99)00047-0

Isolation and characterization of a mouse SRY-related cDNA, mSox7

Koji Taniguchi, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Susumu Kido, Sadakazu Aiso

SIT総合研究所

研究成果: Article

33 引用（Scopus）

抜粋

SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

元の言語	English
ページ（範囲）	225-231
ページ数	7
ジャーナル	Biochimica et Biophysica Acta - Gene Structure and Expression
巻	1445
発行部数	2
DOI	https://doi.org/10.1016/S0167-4781(99)00047-0
出版物ステータス	Published - 1999 5 14

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

文献にアクセス

10.1016/S0167-4781(99)00047-0

フィンガープリント Isolation and characterization of a mouse SRY-related cDNA, mSox7' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

これを引用

Taniguchi, K., Hiraoka, Y., Ogawa, M., Sakai, Y., Kido, S., & Aiso, S. (1999). Isolation and characterization of a mouse SRY-related cDNA, mSox7. Biochimica et Biophysica Acta - Gene Structure and Expression, 1445(2), 225-231. https://doi.org/10.1016/S0167-4781(99)00047-0

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abstract = "SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.",

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AU - Taniguchi, Koji

AU - Hiraoka, Yoshiki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Kido, Susumu

AU - Aiso, Sadakazu

PY - 1999/5/14

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AB - SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

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