Isolation and characterization of a mouse SRY-related cDNA, mSox7

Koji Taniguchi; Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Susumu Kido; Sadakazu Aiso

doi:10.1016/S0167-4781(99)00047-0

Isolation and characterization of a mouse SRY-related cDNA, mSox7

Koji Taniguchi, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Susumu Kido, Sadakazu Aiso

研究成果: Article › 査読

35 被引用数 (Scopus)

抄録

SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

本文言語	English
ページ（範囲）	225-231
ページ数	7
ジャーナル	Biochimica et Biophysica Acta - Gene Structure and Expression
巻	1445
号	2
DOI	https://doi.org/10.1016/S0167-4781(99)00047-0
出版ステータス	Published - 1999 5月 14
外部発表	はい

ASJC Scopus subject areas

構造生物学
生物理学
生化学
遺伝学

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10.1016/S0167-4781(99)00047-0

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title = "Isolation and characterization of a mouse SRY-related cDNA, mSox7",

abstract = "SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.",

keywords = "DNA binding, HMG box, Oocyte, SOX, SRY",

author = "Koji Taniguchi and Yoshiki Hiraoka and Motoyuki Ogawa and Yukinao Sakai and Susumu Kido and Sadakazu Aiso",

note = "Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A. and Grants-in-Aid from the Ministry of Education, Science and Culture to S.A. and Y.H., by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.",

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AU - Kido, Susumu

AU - Aiso, Sadakazu

N1 - Funding Information: This work was supported in part by a National Grant-in-Aid for the Establishment of High-Tech Research Center in a Private University from Ministry of Education, Science and Culture to S.A. and Grants-in-Aid from the Ministry of Education, Science and Culture to S.A. and Y.H., by Keio Gakuji Fukuzawa Memorial Funds for Advancement of Education and Research and Keio Gakuji Academic Developmental Funds from Keio University to S.A. and Y.H.

PY - 1999/5/14

Y1 - 1999/5/14

N2 - SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

AB - SOX is a family of SRY-related genes, which encode transcriptional factors involved in development. In this study, we newly isolated and sequenced mouse cDNA clones for mSox7. The mSox7 gene encodes 380 amino acids containing an SRY-type HMG box. Genomic Southern analysis suggested that the mSox7 gene was a single-copy gene. Tissue specific expression of mSox7 was investigated by Northern analysis. The expression was restricted to the ovary and heart, and the size of the transcripts was estimated to be 3.6 knt. Electrophoretic mobility shift assay indicated that recombinant mSox7 polypeptide was capable of binding to a nucleotide sequence, AACAAT. Immunohistochemical study revealed that mSox7 protein was localized in oocytes in the mouse ovary.

KW - DNA binding

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KW - SRY

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Isolation and characterization of a mouse SRY-related cDNA, mSox7

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