Isolation and Developmental Expression of Mitf in Xenopus laevis

Mayuko Kumasaka, Hiraku Sato, Shigeru Sato, Ichiro Yajima, Hiroaki Yamamoto

研究成果: Article

18 引用 (Scopus)

抄録

Mitf (gene for microphthalmia-associated transcription factor) encodes a transcription factor of the basic/helix-loop-helix/leucine-zipper family and is a key regulator during the development of two different types of melanin-producing cell lineages, namely neural crest-derived melanocytes/melanophores, and the retinal pigment epithelium (RPE) differentiated from the outer layer of the eye cup. Mitf-deficient mice show a lack of melanocytes and small eyes caused by abnormal RPE development. An interesting feature of Mitf is the existence of multiple isoforms with different amino termini and their functions in the development of these melanin-producing pigment cells. In this study, we isolated two Mitf homologues (XI/Mitfα and XIMitfβ) and their isoforms from Xenopus laevis. Alignment analysis of the amino acid sequences of the N-termini suggests that these isoforms are homologues of mouse Mitf-M (expressed specifically in the melanocyte lineage) and Mitf-A (strongly expressed in the RPE, although this expression is ubiquitous). In Xenopus, XIMitfα is strongly expressed in the melanophore lineage (especially in premigratory melanoblasts) and the presumptive RPE and the epiphysis, in which melanin-producing cells differentiate in some vertebrates. Conservation of the Mitf isoforms expected to possess specific functions in the development of melanin-producing cells and of the expressions in such cell types in Xenopus suggest that XIMitf plays a central role in the development of melanin-producing cell lineages, and that, as in mice and humans, most of the signaling molecules or transcription factors implicated genetically in the development of melanin-producing cell lineages affect either Mitf expression or its function.

元の言語English
ページ(範囲)107-113
ページ数7
ジャーナルDevelopmental Dynamics
230
発行部数1
DOI
出版物ステータスPublished - 2004 5 1
外部発表Yes

ASJC Scopus subject areas

  • Developmental Biology

これを引用

Isolation and Developmental Expression of Mitf in Xenopus laevis. / Kumasaka, Mayuko; Sato, Hiraku; Sato, Shigeru; Yajima, Ichiro; Yamamoto, Hiroaki.

:: Developmental Dynamics, 巻 230, 番号 1, 01.05.2004, p. 107-113.

研究成果: Article

Kumasaka, M, Sato, H, Sato, S, Yajima, I & Yamamoto, H 2004, 'Isolation and Developmental Expression of Mitf in Xenopus laevis', Developmental Dynamics, 巻. 230, 番号 1, pp. 107-113. https://doi.org/10.1002/dvdy.20019
Kumasaka, Mayuko ; Sato, Hiraku ; Sato, Shigeru ; Yajima, Ichiro ; Yamamoto, Hiroaki. / Isolation and Developmental Expression of Mitf in Xenopus laevis. :: Developmental Dynamics. 2004 ; 巻 230, 番号 1. pp. 107-113.
@article{aba56af97fa74302a6218244aeb11e2b,
title = "Isolation and Developmental Expression of Mitf in Xenopus laevis",
abstract = "Mitf (gene for microphthalmia-associated transcription factor) encodes a transcription factor of the basic/helix-loop-helix/leucine-zipper family and is a key regulator during the development of two different types of melanin-producing cell lineages, namely neural crest-derived melanocytes/melanophores, and the retinal pigment epithelium (RPE) differentiated from the outer layer of the eye cup. Mitf-deficient mice show a lack of melanocytes and small eyes caused by abnormal RPE development. An interesting feature of Mitf is the existence of multiple isoforms with different amino termini and their functions in the development of these melanin-producing pigment cells. In this study, we isolated two Mitf homologues (XI/Mitfα and XIMitfβ) and their isoforms from Xenopus laevis. Alignment analysis of the amino acid sequences of the N-termini suggests that these isoforms are homologues of mouse Mitf-M (expressed specifically in the melanocyte lineage) and Mitf-A (strongly expressed in the RPE, although this expression is ubiquitous). In Xenopus, XIMitfα is strongly expressed in the melanophore lineage (especially in premigratory melanoblasts) and the presumptive RPE and the epiphysis, in which melanin-producing cells differentiate in some vertebrates. Conservation of the Mitf isoforms expected to possess specific functions in the development of melanin-producing cells and of the expressions in such cell types in Xenopus suggest that XIMitf plays a central role in the development of melanin-producing cell lineages, and that, as in mice and humans, most of the signaling molecules or transcription factors implicated genetically in the development of melanin-producing cell lineages affect either Mitf expression or its function.",
keywords = "Melanin, Melanocyte, Melanophore, Microphthalmia, Mitf, Pigment cell, Retinal pigment epithelium, Xenopus laevis",
author = "Mayuko Kumasaka and Hiraku Sato and Shigeru Sato and Ichiro Yajima and Hiroaki Yamamoto",
year = "2004",
month = "5",
day = "1",
doi = "10.1002/dvdy.20019",
language = "English",
volume = "230",
pages = "107--113",
journal = "Developmental Dynamics",
issn = "1058-8388",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Isolation and Developmental Expression of Mitf in Xenopus laevis

AU - Kumasaka, Mayuko

AU - Sato, Hiraku

AU - Sato, Shigeru

AU - Yajima, Ichiro

AU - Yamamoto, Hiroaki

PY - 2004/5/1

Y1 - 2004/5/1

N2 - Mitf (gene for microphthalmia-associated transcription factor) encodes a transcription factor of the basic/helix-loop-helix/leucine-zipper family and is a key regulator during the development of two different types of melanin-producing cell lineages, namely neural crest-derived melanocytes/melanophores, and the retinal pigment epithelium (RPE) differentiated from the outer layer of the eye cup. Mitf-deficient mice show a lack of melanocytes and small eyes caused by abnormal RPE development. An interesting feature of Mitf is the existence of multiple isoforms with different amino termini and their functions in the development of these melanin-producing pigment cells. In this study, we isolated two Mitf homologues (XI/Mitfα and XIMitfβ) and their isoforms from Xenopus laevis. Alignment analysis of the amino acid sequences of the N-termini suggests that these isoforms are homologues of mouse Mitf-M (expressed specifically in the melanocyte lineage) and Mitf-A (strongly expressed in the RPE, although this expression is ubiquitous). In Xenopus, XIMitfα is strongly expressed in the melanophore lineage (especially in premigratory melanoblasts) and the presumptive RPE and the epiphysis, in which melanin-producing cells differentiate in some vertebrates. Conservation of the Mitf isoforms expected to possess specific functions in the development of melanin-producing cells and of the expressions in such cell types in Xenopus suggest that XIMitf plays a central role in the development of melanin-producing cell lineages, and that, as in mice and humans, most of the signaling molecules or transcription factors implicated genetically in the development of melanin-producing cell lineages affect either Mitf expression or its function.

AB - Mitf (gene for microphthalmia-associated transcription factor) encodes a transcription factor of the basic/helix-loop-helix/leucine-zipper family and is a key regulator during the development of two different types of melanin-producing cell lineages, namely neural crest-derived melanocytes/melanophores, and the retinal pigment epithelium (RPE) differentiated from the outer layer of the eye cup. Mitf-deficient mice show a lack of melanocytes and small eyes caused by abnormal RPE development. An interesting feature of Mitf is the existence of multiple isoforms with different amino termini and their functions in the development of these melanin-producing pigment cells. In this study, we isolated two Mitf homologues (XI/Mitfα and XIMitfβ) and their isoforms from Xenopus laevis. Alignment analysis of the amino acid sequences of the N-termini suggests that these isoforms are homologues of mouse Mitf-M (expressed specifically in the melanocyte lineage) and Mitf-A (strongly expressed in the RPE, although this expression is ubiquitous). In Xenopus, XIMitfα is strongly expressed in the melanophore lineage (especially in premigratory melanoblasts) and the presumptive RPE and the epiphysis, in which melanin-producing cells differentiate in some vertebrates. Conservation of the Mitf isoforms expected to possess specific functions in the development of melanin-producing cells and of the expressions in such cell types in Xenopus suggest that XIMitf plays a central role in the development of melanin-producing cell lineages, and that, as in mice and humans, most of the signaling molecules or transcription factors implicated genetically in the development of melanin-producing cell lineages affect either Mitf expression or its function.

KW - Melanin

KW - Melanocyte

KW - Melanophore

KW - Microphthalmia

KW - Mitf

KW - Pigment cell

KW - Retinal pigment epithelium

KW - Xenopus laevis

UR - http://www.scopus.com/inward/record.url?scp=2142765995&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2142765995&partnerID=8YFLogxK

U2 - 10.1002/dvdy.20019

DO - 10.1002/dvdy.20019

M3 - Article

C2 - 15108314

AN - SCOPUS:2142765995

VL - 230

SP - 107

EP - 113

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 1

ER -