Label-free imaging of melanoma with confocal photothermal microscopy: Differentiation between malignant and benign tissue

Takayoshi Kobayashi, Kazuaki Nakata, Ichiro Yajima, Masashi Kato, Hiromichi Tsurui

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Label-free confocal photothermal (CPT) microscopy was utilized for the first time to investigate malignancy in mouse skin cells. Laser diodes (LDs) with 405 nm or 488 nm wavelengths were used as pumps, and a 638 nm LD was used as a probe for the CPT microscope. A Grey Level Cooccurrence Matrix (GLCM) for texture analysis was applied to the CPT images. Nine GLCM parameters were calculated with definite definitions for the intracellular super-resolved CPT images, and the parameters Entropy, Contrast, and Variance were found to be most suited among the nine parameters to discriminate clearly between healthy cells and malignant cells when a 405 nm pump was used. Prominence, Variance, and Shade were most suited when a pump wavelength of 488 nm was used.

本文言語English
論文番号67
ジャーナルBioengineering
5
3
DOI
出版ステータスPublished - 2018 9
外部発表はい

ASJC Scopus subject areas

  • バイオエンジニアリング

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