Long range surface plasmon-coupled fluorescence emission for biosensor applications

Koji Toma, Wolfgang Knoll, Jakub Dostalek

研究成果: Conference contribution

抄録

A biosensor scheme that employs long range surface plasmons (LRSPs) for the efficient excitation and collection of fluorescence light from fluorophore-labeled biomolecules captured in a three-dimensional hydrogel matrix is discussed. This new approach to plasmon-enhanced fluorescence (PEF) is experimentally and theoretically investigated by using the Kretschmann configuration of attenuated total reflection (ATR) method. A layer structure supporting LRSPs that consists of a low refractive index fluoropolymer layer, a thin gold film and a large binding capacity N-isopropylacrylamide (NIPAAm) hydrogel matrix is employed. By using this layer architecture, the extended field of LRSPs can probe the binding of biomolecules at up to micrometer distances from the gold surface. With respect to regular surface plasmon-enhanced fluorescence spectroscopy (SPFS) and surface plasmon-coupled emission (SPCE), a narrower angular distribution of the fluorescence light intensity, larger peak intensity and the excitation and emission at lower angles were observed.

本文言語English
ホスト出版物のタイトル2011 International Workshop on Biophotonics, BIOPHOTONICS 2011
DOI
出版ステータスPublished - 2011
外部発表はい
イベント2011 International Workshop on Biophotonics, BIOPHOTONICS 2011 - Parma, Italy
継続期間: 2011 6月 82011 6月 10

出版物シリーズ

名前2011 International Workshop on Biophotonics, BIOPHOTONICS 2011

Conference

Conference2011 International Workshop on Biophotonics, BIOPHOTONICS 2011
国/地域Italy
CityParma
Period11/6/811/6/10

ASJC Scopus subject areas

  • 生物理学
  • バイオテクノロジー
  • 生体医工学

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