Loss-of-Function Mutations at the Rim of the Funnel of Mechanosensitive Channel MscL

Kenjiro Yoshimura, Takeshi Nomura, Masahiro Sokabe

研究成果: Article査読

59 被引用数 (Scopus)

抄録

MscL is a bacterial mechanosensitive channel that is activated directly by membrane stretch. Although the gene has been cloned and the crystal structure of the closed channel has been defined, how membrane tension causes conformational changes in MscL remains largely unknown. To identify the site where MscL senses membrane tension, we examined the function of the mutants generated by random and scanning mutagenesis. In vitro (patch-clamp) and in vivo (hypoosmotic-shock) experiments showed that when a hydrophilic amino acid replaces one of the hydrophobic residues that are thought to make contact with the membrane lipid near the periplasmic end of the M1 or M2 transmembrane domain, MscL loses the ability to open in response to membrane tension. Hydrophilic (asparagine) substitution of the other residues in the lipid-protein interface did not impair the channel's mechanosensitivity. These observations suggest that the disturbance of the hydrophobic interaction between the membrane lipid and the periplasmic rim of the channel's funnel impairs the function of MscL.

本文言語English
ページ(範囲)2113-2120
ページ数8
ジャーナルBiophysical Journal
86
4
DOI
出版ステータスPublished - 2004 4月
外部発表はい

ASJC Scopus subject areas

  • 生物理学

フィンガープリント

「Loss-of-Function Mutations at the Rim of the Funnel of Mechanosensitive Channel MscL」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル