MALC, a novel microinjection method for loading of macromolecules into cultured neurons

Ryoji Yano, Daisuke Okada, Chan Choo Yap, Kunihiko Mabuchi, Hitoshi Muguruma, Takashi K. Saito

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Microinjection is one of the most useful and important methods in cellular neurobiology. However, direct insertion and retraction of a capillary produce physical stresses inside cells that make it difficult to apply on small and fragile cells, especially the central nervous system (CNS) neurons. In this study, we developed a novel method called MALC (microinjection method assisted with laser activated chromophore) to avoid this disadvantage of the conventional method. In MALC, a capillary is just attached on cell surface, and combination of a newly developed photosensitizer with a laser pointing system makes the plasma membrane permeable to molecules. This novel method can expand the application of the microinjection method and realize molecular manipulations inside CNS neurons.

本文言語English
ページ(範囲)1263-1266
ページ数4
ジャーナルNeuroReport
13
10
DOI
出版ステータスPublished - 2002 7 19

ASJC Scopus subject areas

  • Neuroscience(all)

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