Metabolism of 2α-propoxy-1α,25-dihydroxyvitamin D3 and 2α-(3-hydroxypropoxy)-1α,25-dihydroxyvitamin D3 by human CYP27A1 and CYP24A1

Daisuke Abe, Toshiyuki Sakaki, Tatsuya Kusudo, Atsushi Kittaka, Nozomi Saito, Yoshitomo Suhara, Toshie Fujishima, Hiroaki Takayama, Hiromi Hamamoto, Masaki Kamakura, Miho Ohta, Kuniyo Inouye

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Recently, we demonstrated that some A-ring-modified vitamin D3 analogs had unique biological activity. Of these analogs, 2α-propoxy- 1α,25(OH)2D3 (C3O1) and 2α-(3-hydroxypropoxy)- 1α,25(OH)2D3 (O2C3) were examined for metabolism by CYP27A1 and CYP24A1. Surprisingly, CYP27A1 catalyzed the conversion from C3O1 to O2C3, which has 3 times more affinity for vitamin D receptor than C3O1. Thus, the conversion from C3O1 to O2C3 by CYP27A1 is considered to be a metabolic activation process. Five metabolites were detected in the metabolism of C3O1 and O2C3 by human CYP24A1 including both C-23 and C-24 oxidation pathways. On the other hand, three metabolites of the C-24 oxidation pathway were detected in their metabolism by rat CYP24A1, indicating a species-based difference in the CYP24A1-dependent metabolism of C3O1 and O2C3 between humans and rats. Kinetic analysis revealed that the Km and kcat values of human CYP24A1 for O2C3 are, respectively, approximately 16 times more and 3 times less than those for 1α,25(OH)2D3. Thus, the catalytic efficiency, kcat/Km, of human CYP24A1 for O2C3 is only 2% of 1α,25(OH)2D3. These results and a high calcium effect of C3O1 and O2C3 in animal experiments using rats suggest that C3O1 and O2C3 are promising for clinical treatment of osteoporosis.

本文言語English
ページ(範囲)778-784
ページ数7
ジャーナルDrug Metabolism and Disposition
33
6
DOI
出版ステータスPublished - 2005 6 1
外部発表はい

ASJC Scopus subject areas

  • 薬理学
  • 薬科学

フィンガープリント

「Metabolism of 2α-propoxy-1α,25-dihydroxyvitamin D<sub>3</sub> and 2α-(3-hydroxypropoxy)-1α,25-dihydroxyvitamin D<sub>3</sub> by human CYP27A1 and CYP24A1」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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