Microfluidic culture of primary neurons with on-chip hypoxic conditioning

Atsushi Takano, Saori Inomata, Masato Tanaka, Nobuyuki Futai

研究成果: Conference contribution

抄録

We have developed a pocket-sized microfluidic cell culture chip that enables on-chip multi-gas (CO2 and O2) control without any feedback control or external gas supply. Elevated CO2 levels (∼7%) and hypoxic conditions (O2 levels, ∼5%) were simultaneously achieved on the chip by maintaining the bottom of the chip at 37 °C and filling an adjacent reservoir with a bicarbonate/carbonate/ascorbate solution. We have also successfully cultured primary chick embryo neurons onchip under hypoxic conditions for 3 days. The neurons cultured under hypoxia showed inhibited neurite outgrowth.

本文言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版社Chemical and Biological Microsystems Society
ページ419-421
ページ数3
ISBN(印刷版)9781632666246
出版ステータスPublished - 2013 1 1
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
継続期間: 2013 10 272013 10 31

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
国/地域Germany
CityFreiburg
Period13/10/2713/10/31

ASJC Scopus subject areas

  • バイオエンジニアリング

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