Microfluidic culture of primary neurons with on-chip hypoxic conditioning

Atsushi Takano, Saori Inomata, Masato Tanaka, Nobuyuki Futai

研究成果: Conference contribution

抄録

We have developed a pocket-sized microfluidic cell culture chip that enables on-chip multi-gas (CO2 and O2) control without any feedback control or external gas supply. Elevated CO2 levels (∼7%) and hypoxic conditions (O2 levels, ∼5%) were simultaneously achieved on the chip by maintaining the bottom of the chip at 37 °C and filling an adjacent reservoir with a bicarbonate/carbonate/ascorbate solution. We have also successfully cultured primary chick embryo neurons onchip under hypoxic conditions for 3 days. The neurons cultured under hypoxia showed inhibited neurite outgrowth.

元の言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版者Chemical and Biological Microsystems Society
ページ419-421
ページ数3
1
ISBN(印刷物)9781632666246
出版物ステータスPublished - 2013
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg
継続期間: 2013 10 272013 10 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Freiburg
期間13/10/2713/10/31

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Microfluidics
Neurons
Gas supply
Carbonates
Bicarbonates
Cell culture
Feedback control
Gases

ASJC Scopus subject areas

  • Bioengineering

これを引用

Takano, A., Inomata, S., Tanaka, M., & Futai, N. (2013). Microfluidic culture of primary neurons with on-chip hypoxic conditioning. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (巻 1, pp. 419-421). Chemical and Biological Microsystems Society.

Microfluidic culture of primary neurons with on-chip hypoxic conditioning. / Takano, Atsushi; Inomata, Saori; Tanaka, Masato; Futai, Nobuyuki.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1 Chemical and Biological Microsystems Society, 2013. p. 419-421.

研究成果: Conference contribution

Takano, A, Inomata, S, Tanaka, M & Futai, N 2013, Microfluidic culture of primary neurons with on-chip hypoxic conditioning. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻. 1, Chemical and Biological Microsystems Society, pp. 419-421, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, 13/10/27.
Takano A, Inomata S, Tanaka M, Futai N. Microfluidic culture of primary neurons with on-chip hypoxic conditioning. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1. Chemical and Biological Microsystems Society. 2013. p. 419-421
Takano, Atsushi ; Inomata, Saori ; Tanaka, Masato ; Futai, Nobuyuki. / Microfluidic culture of primary neurons with on-chip hypoxic conditioning. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1 Chemical and Biological Microsystems Society, 2013. pp. 419-421
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