Molecular cloning and expression of Xenopus laevis Requiem cDNA

Masahiro Konishi; Yoshiki Hiraoka; Motoyuki Ogawa; Yukinao Sakai; Hiromasa Ishii; Sadakazu Aiso

doi:10.1016/S0167-4781(99)00031-7

Molecular cloning and expression of Xenopus laevis Requiem cDNA

Masahiro Konishi, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Hiromasa Ishii, Sadakazu Aiso

研究成果: Article › 査読

5 被引用数 (Scopus)

抄録

Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.

本文言語	English
ページ（範囲）	172-176
ページ数	5
ジャーナル	Biochimica et Biophysica Acta - Gene Structure and Expression
巻	1445
号	1
DOI	https://doi.org/10.1016/S0167-4781(99)00031-7
出版ステータス	Published - 1999 4 14
外部発表	はい

ASJC Scopus subject areas

構造生物学
生物理学
生化学
遺伝学

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10.1016/S0167-4781(99)00031-7

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abstract = "Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.",

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AU - Konishi, Masahiro

AU - Hiraoka, Yoshiki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Ishii, Hiromasa

AU - Aiso, Sadakazu

PY - 1999/4/14

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AB - Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.

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Molecular cloning and expression of Xenopus laevis Requiem cDNA

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