Molecular cloning and expression of Xenopus laevis Requiem cDNA

Masahiro Konishi, Yoshiki Hiraoka, Motoyuki Ogawa, Yukinao Sakai, Hiromasa Ishii, Sadakazu Aiso

研究成果: Article

5 引用 (Scopus)

抄録

Requiem is an apoptosis-associated gene, which was originally identified in mouse (T.G. Gabig et al., J. Biol. Chem. 269 (1994) 29515-29519). In this study, we isolated five independent cDNA clones for frog Requiem (xReq) from Xenopus laevis ovary. Sequence analysis of the multiple cDNAs has suggested that Xenopus genome contains at least two non-allelic copies of the xReq gene. The amino acid sequence deduced from the cDNAs contained a single Kruppel-type zinc-finger motif and two PHD-finger motifs. Northern analysis revealed that the ovary expressed xReq transcripts with different sizes.

元の言語English
ページ(範囲)172-176
ページ数5
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1445
発行部数1
DOI
出版物ステータスPublished - 1999 4 14
外部発表Yes

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Cloning
Complementary DNA
Genes
Zinc
Apoptosis
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

これを引用

Molecular cloning and expression of Xenopus laevis Requiem cDNA. / Konishi, Masahiro; Hiraoka, Yoshiki; Ogawa, Motoyuki; Sakai, Yukinao; Ishii, Hiromasa; Aiso, Sadakazu.

:: Biochimica et Biophysica Acta - Gene Structure and Expression, 巻 1445, 番号 1, 14.04.1999, p. 172-176.

研究成果: Article

Konishi, Masahiro ; Hiraoka, Yoshiki ; Ogawa, Motoyuki ; Sakai, Yukinao ; Ishii, Hiromasa ; Aiso, Sadakazu. / Molecular cloning and expression of Xenopus laevis Requiem cDNA. :: Biochimica et Biophysica Acta - Gene Structure and Expression. 1999 ; 巻 1445, 番号 1. pp. 172-176.
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