Multiphase-laden gas-liquid interface injection for the versatile gene transfer

H. Kuriki, S. Takasawa, M. Iwabuchi, K. Ohsumi, T. Suzuki, T. Higashiyama, S. Sakuma, F. Arai, Y. Yamanishi

研究成果: Conference contribution

2 引用 (Scopus)

抜粋

We have successfully carried out gene transfer using electrically induced bubble knife. The air-liquid interfaces of the dispensed bubbles have novel characteristics which can carry the various kinds of the materials in different phase and viscosity of solution. This technique has advantages in high efficiency of injection of limited amount of valuable samples such as designed m-RNA. In this paper, we demonstrated m-RNA encoding 3xFLAG-GST into xenopus oocyte to be sure that electrically induced bubble knife has ability to injection chemically unstable and biocompatibility. Furthermore, we injected fluorescent beads whose size was 2.1 μm into BY-2 cell as multiphase injection.

元の言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版者Chemical and Biological Microsystems Society
ページ1173-1175
ページ数3
ISBN(印刷物)9781632666246
出版物ステータスPublished - 2013 1 1
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
継続期間: 2013 10 272013 10 31

出版物シリーズ

名前17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
2

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Germany
Freiburg
期間13/10/2713/10/31

ASJC Scopus subject areas

  • Bioengineering

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  • これを引用

    Kuriki, H., Takasawa, S., Iwabuchi, M., Ohsumi, K., Suzuki, T., Higashiyama, T., Sakuma, S., Arai, F., & Yamanishi, Y. (2013). Multiphase-laden gas-liquid interface injection for the versatile gene transfer. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1173-1175). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; 巻数 2). Chemical and Biological Microsystems Society.