Stroke is one of the leading causes of disability in the world. The patient needs a repetitive and consistent rehabilitation treatment in order to get a chance for recovery. Unfortunately, the rural patients have difficulties to perform such treatment due to the transportation problem where they need to travel far away from the remote areas. Therefore, a compact, transportable, and simple operation of device was developed to support the treatment. However, the effect of newly developed rehabilitation device toward human muscles is still unknown due to the limited and restricted testing using real patient. In this research, the newly developed device was evaluated using ergonomic software called AnyBody. Four different movements by using the device were simulated, and six main muscles were selected for analysis. The result shows that different movement using the rehabilitation device gives different effects toward human muscles.