抄録
We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 1681-1683 |
| ページ数 | 3 |
| ジャーナル | Applied Optics |
| 巻 | 36 |
| 発行部数 | 7 |
| DOI | |
| 出版物ステータス | Published - 1997 3 1 |
| 外部発表 | Yes |
Fingerprint
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
これを引用
Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control. / Naya, M.; Micheletto, R.; Mononobe, S.; Umamaheswari, Rajagopalan; Ohtsu, M.
：: Applied Optics, 巻 36, 番号 7, 01.03.1997, p. 1681-1683.研究成果: Article
}
TY - JOUR
T1 - Near-field optical imaging of flagellar filaments of salmonella in water with optical feedback control
AU - Naya, M.
AU - Micheletto, R.
AU - Mononobe, S.
AU - Umamaheswari, Rajagopalan
AU - Ohtsu, M.
PY - 1997/3/1
Y1 - 1997/3/1
N2 - We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.
AB - We report on the high-resolution observation of biological samples in water with a collection-mode near-field optical microscope (c-mode NOM) operating under optical feedback control. With rapidly decreasing evanescent field power used as the feedback signal, for the first time to our knowledge, an image of straight-type flagellar filaments of salmonella in water has been obtained. The estimated diameter of a single filament is around 55 nm with a pixel size of 10 nm. A comparison with its nominal value of 25 nm obtained from electron microscope observations under high vacuum confirms that our c-mode NOM performs high-resolution imaging in water.
KW - Biological samples in water
KW - Collection mode
KW - Evanescent field
KW - Near-field optical microscope
KW - Optical feedback
UR - http://www.scopus.com/inward/record.url?scp=0008605231&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0008605231&partnerID=8YFLogxK
U2 - 10.1364/AO.36.001681
DO - 10.1364/AO.36.001681
M3 - Article
C2 - 18250854
AN - SCOPUS:0008605231
VL - 36
SP - 1681
EP - 1683
JO - Applied Optics
JF - Applied Optics
SN - 0003-6935
IS - 7
ER -