Observation of photoactive yellow protein anchored to a modified Au(1 1 1) surface by scanning tunneling microscopy

Izabela I. Rzeźnicka, George W.H. Wurpel, Mischa Bonn, Michael A. van der Horst, Klaas J. Hellingwerf, Soichiro Matsunaga, Taro Yamada, Maki Kawai

研究成果: Article査読

7 被引用数 (Scopus)

抄録

The adsorption of photoactive yellow protein (PYP) on a Au(1 1 1) surface and its fluorescence activity have been studied by electrochemical scanning tunneling microscopy (EC-STM) and fluorescence photometry. A stable, densely packed protein layer was observed after protein immobilization onto a Au(1 1 1) surface modified with a mixture of 3-mercaptopropanoic acid (3-MPA) and 11-mercaptoundecanoic acid (11-MUA) and subsequent formation of the amide bond with the use of N-hydroxysuccinimide and carbodiimide. Fluorescence photometry data indicate that covalent binding of PYP to the functionalized Au(1 1 1) surface does not interfere with the fluorescence properties of the native protein.

本文言語English
ページ(範囲)113-117
ページ数5
ジャーナルChemical Physics Letters
472
1-3
DOI
出版ステータスPublished - 2009 4月 6
外部発表はい

ASJC Scopus subject areas

  • 物理学および天文学(全般)
  • 物理化学および理論化学

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