抄録
In this paper, we describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the indium-tin-oxide (ITO) microheaters that were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and reduces the SNR. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.
本文言語 | English |
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論文番号 | 5299057 |
ページ(範囲) | 312-317 |
ページ数 | 6 |
ジャーナル | IEEE Transactions on Nanobioscience |
巻 | 8 |
号 | 4 |
DOI | |
出版ステータス | Published - 2009 12月 |
外部発表 | はい |
ASJC Scopus subject areas
- バイオテクノロジー
- バイオエンジニアリング
- 医学(その他)
- 生体医工学
- 薬科学
- コンピュータ サイエンスの応用
- 電子工学および電気工学