On-chip incubation system for long-term microfluidic cell culture

Atsushi Takano, Tomohisa Ogawa, Masato Tanaka, Nobuyuki Futai

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

We demonstrate the use of a microfluidic cell culture chip with Braille pin-driven pumping, capable of on-chip CO2 incubation that does not require an external chamber or gas supply. The proposed chip consists of a poly(dimethylsiloxane)(PDMS)-made microfluidic chip, flip-mounted on a glass slide, that contains a nested pair of cell culture media reservoirs and water-jacket, insulated by a permeable PDMS wall. By using 0.8 M sodium bicarbonate with 65 mM sodium carbonate as the water-jacket and placing on a 37 °C surface, the chip maintained osmolality shift and the pCO2 in the media reservoir stabilized within < 3 mmol/kg and 5.0% ± 0.2% over at least 24 hours. The incubation capabilities were demonstrated through microfluidic culture of CV-1 epithelial cells under an inverted microscope for at least 12 days.

本文言語English
ホスト出版物のタイトル33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
ページ8404-8407
ページ数4
DOI
出版ステータスPublished - 2011 12 26
外部発表はい
イベント33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 - Boston, MA, United States
継続期間: 2011 8 302011 9 3

出版物シリーズ

名前Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN(印刷版)1557-170X

Conference

Conference33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
国/地域United States
CityBoston, MA
Period11/8/3011/9/3

ASJC Scopus subject areas

  • 信号処理
  • 生体医工学
  • コンピュータ ビジョンおよびパターン認識
  • 健康情報学

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