On-chip multi-gas incubation for microfluidic cell cultures under hypoxia

Atsushi Takano, Masato Tanaka, Nobuyuki Futai

研究成果査読

11 被引用数 (Scopus)

抄録

We developed a simple system that regulates CO2 and O2 levels within a microfluidic chip. This system enables long-term cell culture under hypoxic conditions without the need of a CO2 incubator or a multi-gas incubator. Hypoxic conditions were generated using a miniature water jacket containing dissolved ascorbate as an oxygen scavenger. Formulations of the water jacket were determined that enables both 5% pCO2 and desired pO2 levels ranging from 5 to 15%. We also cultured PC-12 cells and primary neuronal cells from chick embryos under hypoxia and observed hypoxia-induced cell death and inhibition of neurite outgrowth.

本文言語English
論文番号061101
ジャーナルBiomicrofluidics
8
6
DOI
出版ステータスPublished - 2014 11 25

ASJC Scopus subject areas

  • 生体医工学
  • 材料科学(全般)
  • 凝縮系物理学
  • 流体および伝熱
  • コロイド化学および表面化学

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