On-chip temperature control under suspended microbridge for cell immobilization and culture system

Yoko Yamanishi, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

研究成果: Conference contribution

抜粋

We describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the Indium-tin-oxide (ITO) microheaters which were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and which reduces the S/N ratio. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

元の言語English
ホスト出版物のタイトルProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版者Chemical and Biological Microsystems Society
ページ1683-1685
ページ数3
ISBN(印刷物)9780979806421
出版物ステータスPublished - 2009 1 1
イベント13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
継続期間: 2009 11 12009 11 5

出版物シリーズ

名前Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Korea, Republic of
Jeju
期間09/11/109/11/5

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • これを引用

    Yamanishi, Y., Teramoto, J., Magariyama, Y., Ishihama, A., Fukuda, T., & Arai, F. (2009). On-chip temperature control under suspended microbridge for cell immobilization and culture system. : Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1683-1685). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.