On-chip temperature control under suspended microbridge for cell immobilization and culture system

Yoko Yamanishi, Jun Teramoto, Yukio Magariyama, Akira Ishihama, Toshio Fukuda, Fumihito Arai

研究成果: Conference contribution

抄録

We describe the fabrication of a temperature-controlled microfluidic chip for cell immobilization using a thermosensitive hydrogel of poly (N-isopropylacrylamide) (PNIPAAm). A mixture of PNIPAAm solution, yeast cells, and Calcein-AM fluorescent dye is flowed in the microchannel, and the Indium-tin-oxide (ITO) microheaters which were fabricated by micromachining technology heat a PNIPAAm gel. However, if the gel is very thick, it blocks the observation of the culturing cells and which reduces the S/N ratio. To address this problem, we fabricate a suspended microbridge above the microheater that limits the height of the gel, ensuring that it forms a thin and transparent layer above the heater. Microheaters and suspended biocompatible microbridge are integrated on a chip in which yeast-cell immobilization can be performed by gelation of a PNIPAAm solution.

本文言語English
ホスト出版物のタイトルProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社Chemical and Biological Microsystems Society
ページ1683-1685
ページ数3
ISBN(印刷版)9780979806421
出版ステータスPublished - 2009 1 1
イベント13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
継続期間: 2009 11 12009 11 5

出版物シリーズ

名前Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
国/地域Korea, Republic of
CityJeju
Period09/11/109/11/5

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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