抄録
Endotoxin concentration in dialysate fluid should be frequently monitored. But conventional limulus test needs complicated operation to prevent the contamination by atmospheric endotoxin. We have developed the flow type measurement system using single tube in which sample solution reacted to limulus reagent without contact with atmosphere. The objective of the present study is to gain a sensitivity of the system to measure the endotoxin concentration ranging under 100 EU-1 accurately. Batch reaction experiments and flow type measurements were performed to determine the optimal wavelength and the reaction time for optimal operation of the continuous system. The highest sensitivity is obtained at a wavelength of 381 nm for measuring the endotoxin concentration by limulus reagent. Proper reaction time differed depending on concentration range of samples. The sensitivity of measuring the endotoxin concentration ranging under 100 EU-1 increased with reaction time. But longer reaction time in the flow type measurement system produces dispersion of the limulus reagent and lower sensitivity. Optimal reaction time of the flow type measurement system is then 45 min. Handy and exact measurements of the endotoxin concentration ranging above 50 EU-1 were able by the condition of a wavelength of 381 nm and a reaction time of 45 min.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 98-101 |
| ページ数 | 4 |
| ジャーナル | Japanese Journal of Artificial Organs |
| 巻 | 25 |
| 発行部数 | 1 |
| 出版物ステータス | Published - 1996 |
| 外部発表 | Yes |
ASJC Scopus subject areas
- Biophysics
これを引用
Optimal design of a continuous measurement system for endotoxin concentration in dialysate fluid. / Mizumoto, D.; Yoshimi, Yasuo; Sakai, K.; Aketagawa, J.; Tanaka, S.
：: Japanese Journal of Artificial Organs, 巻 25, 番号 1, 1996, p. 98-101.研究成果: Article
}
TY - JOUR
T1 - Optimal design of a continuous measurement system for endotoxin concentration in dialysate fluid
AU - Mizumoto, D.
AU - Yoshimi, Yasuo
AU - Sakai, K.
AU - Aketagawa, J.
AU - Tanaka, S.
PY - 1996
Y1 - 1996
N2 - Endotoxin concentration in dialysate fluid should be frequently monitored. But conventional limulus test needs complicated operation to prevent the contamination by atmospheric endotoxin. We have developed the flow type measurement system using single tube in which sample solution reacted to limulus reagent without contact with atmosphere. The objective of the present study is to gain a sensitivity of the system to measure the endotoxin concentration ranging under 100 EU-1 accurately. Batch reaction experiments and flow type measurements were performed to determine the optimal wavelength and the reaction time for optimal operation of the continuous system. The highest sensitivity is obtained at a wavelength of 381 nm for measuring the endotoxin concentration by limulus reagent. Proper reaction time differed depending on concentration range of samples. The sensitivity of measuring the endotoxin concentration ranging under 100 EU-1 increased with reaction time. But longer reaction time in the flow type measurement system produces dispersion of the limulus reagent and lower sensitivity. Optimal reaction time of the flow type measurement system is then 45 min. Handy and exact measurements of the endotoxin concentration ranging above 50 EU-1 were able by the condition of a wavelength of 381 nm and a reaction time of 45 min.
AB - Endotoxin concentration in dialysate fluid should be frequently monitored. But conventional limulus test needs complicated operation to prevent the contamination by atmospheric endotoxin. We have developed the flow type measurement system using single tube in which sample solution reacted to limulus reagent without contact with atmosphere. The objective of the present study is to gain a sensitivity of the system to measure the endotoxin concentration ranging under 100 EU-1 accurately. Batch reaction experiments and flow type measurements were performed to determine the optimal wavelength and the reaction time for optimal operation of the continuous system. The highest sensitivity is obtained at a wavelength of 381 nm for measuring the endotoxin concentration by limulus reagent. Proper reaction time differed depending on concentration range of samples. The sensitivity of measuring the endotoxin concentration ranging under 100 EU-1 increased with reaction time. But longer reaction time in the flow type measurement system produces dispersion of the limulus reagent and lower sensitivity. Optimal reaction time of the flow type measurement system is then 45 min. Handy and exact measurements of the endotoxin concentration ranging above 50 EU-1 were able by the condition of a wavelength of 381 nm and a reaction time of 45 min.
KW - Endotoxin
KW - Flow type measurement system
UR - http://www.scopus.com/inward/record.url?scp=0029923455&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029923455&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0029923455
VL - 25
SP - 98
EP - 101
JO - Japanese Journal of Artificial Organs
JF - Japanese Journal of Artificial Organs
SN - 0300-0818
IS - 1
ER -