Optimisation of biodegradation conditions for cyanide removal by Serratia marcescens strain AQ07 using one-factor-at-a-time technique and response surface methodology

Kabiru Ibrahim Karamba, Siti Aqlima Ahmad, Azham Zulkharnain, Mohd Arif Syed, Khalilah Abdul Khalil, Nor Aripin Shamaan, Farrah Aini Dahalan, Mohd Yunus Shukor

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Gold mining companies are known to use cyanide to extract gold from minerals. The indiscriminate use of cyanide presents a major environmental issue. Serratia marcescens strain AQ07 was found to have cyanide-degrading ability. Optimisation of biodegradation condition was carried out utilising one factor at a time and response surface methodology. Cyanide degradation corresponded with growth rate with a maximum growth rate of 16.14 log cfu/mL on day 3 of incubation. Glucose and yeast extract are suitable carbon and nitrogen sources. Six parameters including carbon and nitrogen sources, pH, temperature, inoculum size and cyanide concentration were optimised. In line with the central composite design of response surface methodology, cyanide degradation was optimum at glucose concentration 5.5 g/L, yeast extract 0.55 g/L, pH 6, temperature 32.5 °C, inoculum size 20 % and cyanide concentration 200 mg/L. It was able to stand cyanide toxicity of up to 700 mg/L, which makes it an important candidate for bioremediation of cyanide. The bacterium was observed to degrade 95.6 % of 200 mg/L KCN under the optimised condition. Bacteria are reported to degrade cyanide into ammonia, formamide or formate and carbon dioxide, which are less toxic by-products. These bacteria illustrate good cyanide degradation potential that can be harnessed in cyanide remediation.

本文言語English
ページ(範囲)533-545
ページ数13
ジャーナルRendiconti Lincei
27
3
DOI
出版ステータスPublished - 2016 9 1
外部発表はい

ASJC Scopus subject areas

  • 環境科学(全般)
  • 農業および生物科学(全般)
  • 地球惑星科学(全般)

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