Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest

Megumi Kurita, Hiroaki Suzuki, Hisao Masai, Kiyohisa Mizumoto, Etsuro Ogata, Ikuo Nishimoto, Sadakazu Aiso, Masaaki Matsuoka

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Cdc7 expression repressor (CR)/periphilin has been originally cloned as an interactor with periplakin, a precursor of the cornified cell envelope, and suggested to constitute a new type of nuclear matrix. We here show that CR/periphilin is a ubiquitously expressed nuclear protein with speckled distribution. Overexpression of CR/periphilin induces S-phase arrest. Analysis of expression of regulators involved in DNA replication has revealed that both mRNA and protein expression of Cdc7, a regulator of the initiation and continuation of DNA replication, are markedly downregulated by overexpression of CR/periphilin. However, co-expression of Cdc7 only marginally rescues S-phase arrest induced by CR, indicating that CR retards S-phase progression by modifying expression of some genes including Cdc7, which are involved in progression of DNA replication or coordination of DNA replication and S-phase progression.

本文言語English
ページ(範囲)554-561
ページ数8
ジャーナルBiochemical and Biophysical Research Communications
324
2
DOI
出版ステータスPublished - 2004 11 12
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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