Permanent magnets circuit for collagen orientation

To synthesize artificial bones, the collagen orientation is one of the key issues. It is reported that high magnetic field could be generated by superconducting magnets, are effective in controlling collagen orientation. In this paper, magnetic circuit using permanent magnets for collagen orientation is studied. As the results, a compact magnetic circuit with magnetic field strength around 3 T has been developed. By using the magnetic circuit, collagen was highly oriented, and orientation order was 0.9. Mouse osteoblast cells were seeded on the orientated collagen solution and cultured. The collagen-gel shrinkages by cells were observed. The shrinkage rates of oriented collagen are higher than that of not oriented one.