We developed a novel cell chip, which enables us to control cell adhesion of the bottom face in a micro channel by local light irradiation. A flow-type cell chip was constructed by attaching a sheet of poly (dimethyl siloxane) having micro-channel structure at surface, to a photo-responsive cell culture surface, which was functionalized with poly(N-isopropylacrylamide) containing nitrospiropyran residues, by pressure bonding. After systematic examination of the chip design and cell culture condition, we specified the condition allowing to culture living cells in the micro channel for more than 30 hours. We also confirmed that the irradiation of UV light with the wavelength of 365 nm brought a drastic enhancement of cell adhesion as we confirmed previously in dish-like culture system even in a micro channel of the flow-type chip. Practically we succeeded to introduce living cell only in a required region of a micro channel, and to remove the cells from the other region (Fig.1). Further, by using a fluorescent dye, which stains living cells only, viability of the cells was confirmed to be maintained well even after the procedure of cell adhesion control.
|出版ステータス||Published - 2005 12月 1|
|イベント||54th SPSJ Symposium on Macromolecules - Yamagata, Japan|
継続期間: 2005 9月 20 → 2005 9月 22
|Conference||54th SPSJ Symposium on Macromolecules|
|Period||05/9/20 → 05/9/22|
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