Photo-control of cell adhesion in a flow-type cell chip

Yuichi Tada, Kimio Sumaru, Shinji Sugiura, Junichi Edahiro, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

研究成果: Paper

抜粋

We developed a novel cell chip, which enables us to control cell adhesion of the bottom face in a micro channel by local light irradiation. A flow-type cell chip was constructed by attaching a sheet of poly (dimethyl siloxane) having micro-channel structure at surface, to a photo-responsive cell culture surface, which was functionalized with poly(N-isopropylacrylamide) containing nitrospiropyran residues, by pressure bonding. After systematic examination of the chip design and cell culture condition, we specified the condition allowing to culture living cells in the micro channel for more than 30 hours. We also confirmed that the irradiation of UV light with the wavelength of 365 nm brought a drastic enhancement of cell adhesion as we confirmed previously in dish-like culture system even in a micro channel of the flow-type chip. Practically we succeeded to introduce living cell only in a required region of a micro channel, and to remove the cells from the other region (Fig.1). Further, by using a fluorescent dye, which stains living cells only, viability of the cells was confirmed to be maintained well even after the procedure of cell adhesion control.

元の言語English
ページ数1
出版物ステータスPublished - 2005 12 1
イベント54th SPSJ Symposium on Macromolecules - Yamagata, Japan
継続期間: 2005 9 202005 9 22

Conference

Conference54th SPSJ Symposium on Macromolecules
Japan
Yamagata
期間05/9/2005/9/22

ASJC Scopus subject areas

  • Engineering(all)

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  • これを引用

    Tada, Y., Sumaru, K., Sugiura, S., Edahiro, J., Takagi, T., Shinbo, T., Kanamori, T., & Yoshimi, Y. (2005). Photo-control of cell adhesion in a flow-type cell chip. 論文発表場所 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.