Photogeneration of NADPH by oligothiophenes coupled with ferredoxin- NADP reductase

Y. Kim, K. Ikebukuro, H. Muguruma, I. Karube

研究成果: Article

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The photogeneration of nicotinamide adenine dinucleotide hydrophosphate (NADPH) and its associated reaction were studied in a novel photo-energy conversion system. The system was composed of the oligothiophene, dimethyl- 4,4'-bipyridinium (MV2+), EDTA, and combined with ferredoxin-NADP reductase (FDR, E.C.1.18.1.2). The NADPH was generated by the FDR catalysis via the reduction of MV2+ by the photocatalysis of oligothiophene and EDTA as the sacrificial electron donor. As a result, the rate of the NADPH generation depended on the concentrations of nicotinamide adenine dinucleotide phosphate (NADP+) and MV2+. The effect of the intension of applied light showed the same tendency. Rate values of photoreduction of MV2+ and photogeneration of NADPH systems were investigated as a function of the concentration of MV2+. The production rates of methyl viologen radical cation (MV+) were a 1000- fold more than the NADPH production rates in the oligothiophenes. These results suggest that the electron transfer between FDR and its substrates is the rate-determining step.

元の言語English
ページ(範囲)213-220
ページ数8
ジャーナルJournal of Biotechnology
59
発行部数3
DOI
出版物ステータスPublished - 1998 1 3

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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