Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen

Kayo Ohkouchi, Seiji Kawamoto, Kenji Tatsugawa, Noboru Yoshikawa, Yuki Takaoka, Sayumi Miyauchi, Tsunehiro Aki, Mitsuo Yamashita, Yoshikatsu Murooka, Kazuhisa Ono

研究成果: Article

15 引用 (Scopus)

抜粋

Lactic acid bacteria (LAB) represent an attractive delivery vehicle for oral allergy vaccine because of their safety as a food microorganism as well as their potent adjuvant activity triggering anti-allergic immune response. Here, we report the generation of recombinant LAB expressing a major Japanese cedar pollen allergen Cry j 1 (Cry j 1-LAB), and their prophylactic effect in vivo. To facilitate heterologous expression, the codon usage in the Cry j 1 gene was optimized for the host LAB strain Lactobacillus plantarum by the recursive PCR-based exhaustive site-directed mutagenesis. Use of the codon-optimized Cry j 1 cDNA and a lactate dehydrogenase gene fusion system led to a successful production of recombinant Cry j 1 in L. plantarum NCL21. We also found that oral vaccination with the Cry j 1-LAB suppressed allergen-specific IgE response and nasal symptoms in a murine model of cedar pollinosis.

元の言語English
ページ(範囲)536-541
ページ数6
ジャーナルJournal of Bioscience and Bioengineering
113
発行部数4
DOI
出版物ステータスPublished - 2012 4 1

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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  • これを引用

    Ohkouchi, K., Kawamoto, S., Tatsugawa, K., Yoshikawa, N., Takaoka, Y., Miyauchi, S., Aki, T., Yamashita, M., Murooka, Y., & Ono, K. (2012). Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen. Journal of Bioscience and Bioengineering, 113(4), 536-541. https://doi.org/10.1016/j.jbiosc.2011.11.025