Quantification of fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry

Maya Kamao, Naoko Tsugawa, Yoshitomo Suhara, Akimori Wada, Toshiyuki Mori, Kazuo Murata, Riichiro Nishino, Tetsuya Ukita, Kazuhiro Uenishi, Kiyoshi Tanaka, Toshio Okano

研究成果: Article

80 引用 (Scopus)

抄録

Sensitive quantification method for fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry was developed. Vitamins A, D and E were extracted from 10.0 mL of breast milk after saponifying by basic condition. Vitamin K derivatives were extracted from 3.0 mL of breast milk after lipase treatment. The corresponding stable isotope-labeled compounds were used as internal standards. For the determination of vitamin D compounds, derivatization with a Cookson-type reagent was performed. All fat-soluble vitamins were determined by liquid chromatography-tandem mass spectrometry in the positive ion mode. The detection limits of all analytes were 1-250 pg per 50 μL. The recoveries of fat-soluble vitamins were 91-105%. Inter-assay CV values of each vitamin were 1.9-11.9%. The mean concentrations of retinol, vitamin D3, 25-hydroxyvitamin D3, α-tocopherol, phylloquinone and menaquinone-4 were 0.455 μg/mL, 0.088 ng/mL, 0.081 ng/mL, 5.087 μg/mL, 3.771 ng/mL, and 1.795 ng/mL, respectively (n = 82). This method makes possible to determine fat-soluble vitamins with a wide range of polarities in human breast milk. The assay may be useful for large-scale studies.

元の言語English
ページ(範囲)192-200
ページ数9
ジャーナルJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
859
発行部数2
DOI
出版物ステータスPublished - 2007 11 15
外部発表Yes

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Liquid chromatography
Vitamins
Mass spectrometry
Fats
Vitamin A
Assays
Vitamin K 1
Calcifediol
Tocopherols
Vitamin K
Cholecalciferol
Lipase
Vitamin D
Isotopes
Positive ions
Human Milk
Derivatives
Recovery

ASJC Scopus subject areas

  • Biochemistry

これを引用

Quantification of fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry. / Kamao, Maya; Tsugawa, Naoko; Suhara, Yoshitomo; Wada, Akimori; Mori, Toshiyuki; Murata, Kazuo; Nishino, Riichiro; Ukita, Tetsuya; Uenishi, Kazuhiro; Tanaka, Kiyoshi; Okano, Toshio.

:: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 巻 859, 番号 2, 15.11.2007, p. 192-200.

研究成果: Article

Kamao, Maya ; Tsugawa, Naoko ; Suhara, Yoshitomo ; Wada, Akimori ; Mori, Toshiyuki ; Murata, Kazuo ; Nishino, Riichiro ; Ukita, Tetsuya ; Uenishi, Kazuhiro ; Tanaka, Kiyoshi ; Okano, Toshio. / Quantification of fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry. :: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2007 ; 巻 859, 番号 2. pp. 192-200.
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AU - Tsugawa, Naoko

AU - Suhara, Yoshitomo

AU - Wada, Akimori

AU - Mori, Toshiyuki

AU - Murata, Kazuo

AU - Nishino, Riichiro

AU - Ukita, Tetsuya

AU - Uenishi, Kazuhiro

AU - Tanaka, Kiyoshi

AU - Okano, Toshio

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N2 - Sensitive quantification method for fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry was developed. Vitamins A, D and E were extracted from 10.0 mL of breast milk after saponifying by basic condition. Vitamin K derivatives were extracted from 3.0 mL of breast milk after lipase treatment. The corresponding stable isotope-labeled compounds were used as internal standards. For the determination of vitamin D compounds, derivatization with a Cookson-type reagent was performed. All fat-soluble vitamins were determined by liquid chromatography-tandem mass spectrometry in the positive ion mode. The detection limits of all analytes were 1-250 pg per 50 μL. The recoveries of fat-soluble vitamins were 91-105%. Inter-assay CV values of each vitamin were 1.9-11.9%. The mean concentrations of retinol, vitamin D3, 25-hydroxyvitamin D3, α-tocopherol, phylloquinone and menaquinone-4 were 0.455 μg/mL, 0.088 ng/mL, 0.081 ng/mL, 5.087 μg/mL, 3.771 ng/mL, and 1.795 ng/mL, respectively (n = 82). This method makes possible to determine fat-soluble vitamins with a wide range of polarities in human breast milk. The assay may be useful for large-scale studies.

AB - Sensitive quantification method for fat-soluble vitamins in human breast milk by liquid chromatography-tandem mass spectrometry was developed. Vitamins A, D and E were extracted from 10.0 mL of breast milk after saponifying by basic condition. Vitamin K derivatives were extracted from 3.0 mL of breast milk after lipase treatment. The corresponding stable isotope-labeled compounds were used as internal standards. For the determination of vitamin D compounds, derivatization with a Cookson-type reagent was performed. All fat-soluble vitamins were determined by liquid chromatography-tandem mass spectrometry in the positive ion mode. The detection limits of all analytes were 1-250 pg per 50 μL. The recoveries of fat-soluble vitamins were 91-105%. Inter-assay CV values of each vitamin were 1.9-11.9%. The mean concentrations of retinol, vitamin D3, 25-hydroxyvitamin D3, α-tocopherol, phylloquinone and menaquinone-4 were 0.455 μg/mL, 0.088 ng/mL, 0.081 ng/mL, 5.087 μg/mL, 3.771 ng/mL, and 1.795 ng/mL, respectively (n = 82). This method makes possible to determine fat-soluble vitamins with a wide range of polarities in human breast milk. The assay may be useful for large-scale studies.

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KW - Vitamin A

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