Random mutagenesis of pullulanase from Klebsiella aerogenes for studies of the structure and function of the enzyme

Mitsuo Yamashita, Takuya Kinoshita, Michiko Ihara, Tomomi Mikawa, Yoshikatu Murooka

研究成果: Article査読

11 被引用数 (Scopus)

抄録

To study the structure and function of pullulanase from Klebsiella aerogenes, a method involving random mutagenesis of the entire gene for pullulanase was used. Out of 50, 000 clones screened at high temperature, seven genes for mutant proteins were identified by DNA sequencing. The amino acid substitutions in the seven mutant proteins were clustered on the NH2-terminal side of the four conserved regions found in α-amylases. These mutant pullulanases were classified into two types: those whose catalytic activity was altered and those whose thermal stability was increased. The results presented here and in previous reports suggest that pullulanase from K. aerogenes has similar active sites to those of α-amylases with the four conserved regions, as well as another substrate-binding site closer to the NH2-terminus. The plate assay method used for isolation of thermostable variants may be applicable to the generation of useful variants of other enzymes.

本文言語English
ページ(範囲)1233-1246
ページ数14
ジャーナルJournal of biochemistry
116
6
DOI
出版ステータスPublished - 1994 12月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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