Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel

Akira Yamaguchi, Peng Jin, Hiroshi Tsuchiyama, Takao Masuda, Kai Sun, Shigeki Matsuo, Hiroaki Misawa

研究成果: Article

41 引用 (Scopus)

抄録

Applicability of polydimethylsiloxane (PDMS) for easy and rapid fabrication of enzyme sensor chips, based on electrochemical detection, is examined. The sensor chip consists of PDMS substrate with a microfluidic channel fabricated in it, and a glass substrate with enzyme-modified microelectrodes. The two substrates are clamped together between plastic plates. The sensor chip has shown no leakage around the microelectrodes under continuous solution flow (34μl/min). Amperometric response of the sensor chips developed in this work suggest that various types of enzyme sensors can be designed by using PDMS microfluidic channels.

元の言語English
ページ(範囲)143-152
ページ数10
ジャーナルAnalytica Chimica Acta
468
発行部数1
DOI
出版物ステータスPublished - 2002 9 10
外部発表Yes

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Enzyme sensors
Electrochemical sensors
Microfluidics
Microelectrodes
enzyme
sensor
Fabrication
Sensors
Substrates
substrate
Leakage (fluid)
Plastics
Glass
leakage
Enzymes
glass
plastic
baysilon

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

これを引用

Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel. / Yamaguchi, Akira; Jin, Peng; Tsuchiyama, Hiroshi; Masuda, Takao; Sun, Kai; Matsuo, Shigeki; Misawa, Hiroaki.

:: Analytica Chimica Acta, 巻 468, 番号 1, 10.09.2002, p. 143-152.

研究成果: Article

Yamaguchi, Akira ; Jin, Peng ; Tsuchiyama, Hiroshi ; Masuda, Takao ; Sun, Kai ; Matsuo, Shigeki ; Misawa, Hiroaki. / Rapid fabrication of electrochemical enzyme sensor chip using polydimethylsiloxane microfluidic channel. :: Analytica Chimica Acta. 2002 ; 巻 468, 番号 1. pp. 143-152.
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