抄録
We report a new aspect of rapid (< 30 seconds) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue and a monomer of conducting polymer, using a high-power laser (light intensity 1.6 W cm -2). A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to insertion. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Three toxic processes of the cells by BAT can be identified: the first is a non-light-mediated toxicity dependent on BAT exposure time; the second is a phototoxicity independent of BAT exposure time when the BAT concentration is 2 - 10 μM range; and the third is a phototoxicity dependent on BAT exposure time with 20 μM of BAT concentration in case that cytotoxicity decreases when α-tocopherol, an anti-oxidant, is added to a cell membrane. This is the typical phototoxicity that the phospholipid peroxidation chain reaction triggered by a reactive oxygen species generated by a triplet state of BAT.
| 元の言語 | English |
|---|---|
| ホスト出版物のタイトル | Annual Reports of the Research Reactor Institute, Kyoto University |
| ページ | 2953-2956 |
| ページ数 | 4 |
| 巻 | 3 |
| 出版物ステータス | Published - 2001 |
| 外部発表 | Yes |
| イベント | 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Istanbul, Turkey 継続期間: 2001 10 25 → 2001 10 28 |
Other
| Other | 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society |
|---|---|
| 国 | Turkey |
| 市 | Istanbul |
| 期間 | 01/10/25 → 01/10/28 |
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ASJC Scopus subject areas
- Energy Engineering and Power Technology
- Mechanical Engineering
これを引用
Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode. / Saito, Takashi K.; Takahashi, Mareyuki; Muguruma, Hitoshi; Suzuki, Takafumi; Kakuta, Naoto; Niki, Etsuo; Mabuchi, Kunihiko.
Annual Reports of the Research Reactor Institute, Kyoto University. 巻 3 2001. p. 2953-2956.研究成果: Conference contribution
}
TY - GEN
T1 - Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode
AU - Saito, Takashi K.
AU - Takahashi, Mareyuki
AU - Muguruma, Hitoshi
AU - Suzuki, Takafumi
AU - Kakuta, Naoto
AU - Niki, Etsuo
AU - Mabuchi, Kunihiko
PY - 2001
Y1 - 2001
N2 - We report a new aspect of rapid (< 30 seconds) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue and a monomer of conducting polymer, using a high-power laser (light intensity 1.6 W cm -2). A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to insertion. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Three toxic processes of the cells by BAT can be identified: the first is a non-light-mediated toxicity dependent on BAT exposure time; the second is a phototoxicity independent of BAT exposure time when the BAT concentration is 2 - 10 μM range; and the third is a phototoxicity dependent on BAT exposure time with 20 μM of BAT concentration in case that cytotoxicity decreases when α-tocopherol, an anti-oxidant, is added to a cell membrane. This is the typical phototoxicity that the phospholipid peroxidation chain reaction triggered by a reactive oxygen species generated by a triplet state of BAT.
AB - We report a new aspect of rapid (< 30 seconds) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue and a monomer of conducting polymer, using a high-power laser (light intensity 1.6 W cm -2). A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to insertion. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Three toxic processes of the cells by BAT can be identified: the first is a non-light-mediated toxicity dependent on BAT exposure time; the second is a phototoxicity independent of BAT exposure time when the BAT concentration is 2 - 10 μM range; and the third is a phototoxicity dependent on BAT exposure time with 20 μM of BAT concentration in case that cytotoxicity decreases when α-tocopherol, an anti-oxidant, is added to a cell membrane. This is the typical phototoxicity that the phospholipid peroxidation chain reaction triggered by a reactive oxygen species generated by a triplet state of BAT.
KW - Membrane
KW - Microelectrode
KW - PC12
KW - Photosensitizer
UR - http://www.scopus.com/inward/record.url?scp=0035782247&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035782247&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:0035782247
VL - 3
SP - 2953
EP - 2956
BT - Annual Reports of the Research Reactor Institute, Kyoto University
ER -