Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode

Takashi K. Saito, Mareyuki Takahashi, Hitoshi Muguruma, Takafumi Suzuki, Naoto Kakuta, Etsuo Niki, Kunihiko Mabuchi

研究成果: Conference contribution

抄録

We report a new aspect of rapid (< 30 seconds) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue and a monomer of conducting polymer, using a high-power laser (light intensity 1.6 W cm -2). A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to insertion. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Three toxic processes of the cells by BAT can be identified: the first is a non-light-mediated toxicity dependent on BAT exposure time; the second is a phototoxicity independent of BAT exposure time when the BAT concentration is 2 - 10 μM range; and the third is a phototoxicity dependent on BAT exposure time with 20 μM of BAT concentration in case that cytotoxicity decreases when α-tocopherol, an anti-oxidant, is added to a cell membrane. This is the typical phototoxicity that the phospholipid peroxidation chain reaction triggered by a reactive oxygen species generated by a triplet state of BAT.

元の言語English
ホスト出版物のタイトルAnnual Reports of the Research Reactor Institute, Kyoto University
ページ2953-2956
ページ数4
3
出版物ステータスPublished - 2001
外部発表Yes
イベント23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society - Istanbul, Turkey
継続期間: 2001 10 252001 10 28

Other

Other23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Turkey
Istanbul
期間01/10/2501/10/28

Fingerprint

Microelectrodes
Thiophene
Cell membranes
Phospholipids
High power lasers
Conducting polymers
Cytotoxicity
Oxidants
Toxicity
Monomers
Membranes
Oxygen
Water

ASJC Scopus subject areas

  • Energy Engineering and Power Technology
  • Mechanical Engineering

これを引用

Saito, T. K., Takahashi, M., Muguruma, H., Suzuki, T., Kakuta, N., Niki, E., & Mabuchi, K. (2001). Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode. : Annual Reports of the Research Reactor Institute, Kyoto University (巻 3, pp. 2953-2956)

Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode. / Saito, Takashi K.; Takahashi, Mareyuki; Muguruma, Hitoshi; Suzuki, Takafumi; Kakuta, Naoto; Niki, Etsuo; Mabuchi, Kunihiko.

Annual Reports of the Research Reactor Institute, Kyoto University. 巻 3 2001. p. 2953-2956.

研究成果: Conference contribution

Saito, TK, Takahashi, M, Muguruma, H, Suzuki, T, Kakuta, N, Niki, E & Mabuchi, K 2001, Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode. : Annual Reports of the Research Reactor Institute, Kyoto University. 巻. 3, pp. 2953-2956, 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Istanbul, Turkey, 01/10/25.
Saito TK, Takahashi M, Muguruma H, Suzuki T, Kakuta N, Niki E その他. Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode. : Annual Reports of the Research Reactor Institute, Kyoto University. 巻 3. 2001. p. 2953-2956
Saito, Takashi K. ; Takahashi, Mareyuki ; Muguruma, Hitoshi ; Suzuki, Takafumi ; Kakuta, Naoto ; Niki, Etsuo ; Mabuchi, Kunihiko. / Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode. Annual Reports of the Research Reactor Institute, Kyoto University. 巻 3 2001. pp. 2953-2956
@inproceedings{899f5b24784049438d2586545fcf2baf,
title = "Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode",
abstract = "We report a new aspect of rapid (< 30 seconds) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue and a monomer of conducting polymer, using a high-power laser (light intensity 1.6 W cm -2). A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to insertion. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Three toxic processes of the cells by BAT can be identified: the first is a non-light-mediated toxicity dependent on BAT exposure time; the second is a phototoxicity independent of BAT exposure time when the BAT concentration is 2 - 10 μM range; and the third is a phototoxicity dependent on BAT exposure time with 20 μM of BAT concentration in case that cytotoxicity decreases when α-tocopherol, an anti-oxidant, is added to a cell membrane. This is the typical phototoxicity that the phospholipid peroxidation chain reaction triggered by a reactive oxygen species generated by a triplet state of BAT.",
keywords = "Membrane, Microelectrode, PC12, Photosensitizer",
author = "Saito, {Takashi K.} and Mareyuki Takahashi and Hitoshi Muguruma and Takafumi Suzuki and Naoto Kakuta and Etsuo Niki and Kunihiko Mabuchi",
year = "2001",
language = "English",
volume = "3",
pages = "2953--2956",
booktitle = "Annual Reports of the Research Reactor Institute, Kyoto University",

}

TY - GEN

T1 - Rapid photosensitive cell membrane damage process of novel thiophene for microelectrode

AU - Saito, Takashi K.

AU - Takahashi, Mareyuki

AU - Muguruma, Hitoshi

AU - Suzuki, Takafumi

AU - Kakuta, Naoto

AU - Niki, Etsuo

AU - Mabuchi, Kunihiko

PY - 2001

Y1 - 2001

N2 - We report a new aspect of rapid (< 30 seconds) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue and a monomer of conducting polymer, using a high-power laser (light intensity 1.6 W cm -2). A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to insertion. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Three toxic processes of the cells by BAT can be identified: the first is a non-light-mediated toxicity dependent on BAT exposure time; the second is a phototoxicity independent of BAT exposure time when the BAT concentration is 2 - 10 μM range; and the third is a phototoxicity dependent on BAT exposure time with 20 μM of BAT concentration in case that cytotoxicity decreases when α-tocopherol, an anti-oxidant, is added to a cell membrane. This is the typical phototoxicity that the phospholipid peroxidation chain reaction triggered by a reactive oxygen species generated by a triplet state of BAT.

AB - We report a new aspect of rapid (< 30 seconds) light-induced cell membrane damage photosensitized by 5,5″-bis(aminomethyl)-2,2′:5′,2″-terthiophene dihydrochloride (BAT), which is a water-soluble α-terthienyl analogue and a monomer of conducting polymer, using a high-power laser (light intensity 1.6 W cm -2). A microelectrode insertion process to cell could be improved by pinpoint use of the photosensitizing (PS) effect to degenerate cell membranes prior to insertion. This avoids mechanical perforation of the membrane when the pipette tip makes contact with the cell. Three toxic processes of the cells by BAT can be identified: the first is a non-light-mediated toxicity dependent on BAT exposure time; the second is a phototoxicity independent of BAT exposure time when the BAT concentration is 2 - 10 μM range; and the third is a phototoxicity dependent on BAT exposure time with 20 μM of BAT concentration in case that cytotoxicity decreases when α-tocopherol, an anti-oxidant, is added to a cell membrane. This is the typical phototoxicity that the phospholipid peroxidation chain reaction triggered by a reactive oxygen species generated by a triplet state of BAT.

KW - Membrane

KW - Microelectrode

KW - PC12

KW - Photosensitizer

UR - http://www.scopus.com/inward/record.url?scp=0035782247&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035782247&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0035782247

VL - 3

SP - 2953

EP - 2956

BT - Annual Reports of the Research Reactor Institute, Kyoto University

ER -