Release of the serum immunosuppressive factor by monocytes in patients with crohn's disease

M. Watanabe, T. Hibi, Y. Iwao, T. Kanai, M. Ohara, T. H. Fujisawa Ogata, N. Watanabe, H. Asakura, Sadakazu Aiso, S. Tsuru, M. Tsuchiya

研究成果: Article

2 引用 (Scopus)

抄録

We investigated the in vitro immunosuppressive effect of the sera and the culture supernatants of the peripheral blood mononuclear cells obtained from patients with Crohn's disease. Sera from Crohn's disease markedly suppressed the proliferative response of mouse spleen cells, compared with sera from ulcerative colitis, intestinal tuberculosis and normal controls. The culture supernatants of the adherent mononuclear cells from Crohn's disease showed a remarkable suppressive effect (96±1%), while the culture supernatants of the nonadherent mononuclear cells ha no suppressive activity (-10±16%). The culture supernatants of the adherent and non-adherent mononuclear cells from normal controls had no suppressive activity. The fractionization of the culture supernatants of the adherent cells from the Crohn's disease patients demonstrated that the fractions with high suppressive activity had similar or identical biochemical properties to the serum immunosuppressive fractions which has been reported previously. These results indicate that monocytes may release the serum immunosuppressive factor in Crohn's disease. This factor may contribute to the depression of the immune reactivity in the mucosal lesion and to the persistence of the stimulation of abnormal immune response in the intestinal wall.

元の言語English
ページ(範囲)161-166
ページ数6
ジャーナルJournal of Clinical and Laboratory Immunology
29
発行部数4
出版物ステータスPublished - 1989 12 1
外部発表Yes

ASJC Scopus subject areas

  • Immunology

これを引用

Watanabe, M., Hibi, T., Iwao, Y., Kanai, T., Ohara, M., Fujisawa Ogata, T. H., ... Tsuchiya, M. (1989). Release of the serum immunosuppressive factor by monocytes in patients with crohn's disease. Journal of Clinical and Laboratory Immunology, 29(4), 161-166.

Release of the serum immunosuppressive factor by monocytes in patients with crohn's disease. / Watanabe, M.; Hibi, T.; Iwao, Y.; Kanai, T.; Ohara, M.; Fujisawa Ogata, T. H.; Watanabe, N.; Asakura, H.; Aiso, Sadakazu; Tsuru, S.; Tsuchiya, M.

:: Journal of Clinical and Laboratory Immunology, 巻 29, 番号 4, 01.12.1989, p. 161-166.

研究成果: Article

Watanabe, M, Hibi, T, Iwao, Y, Kanai, T, Ohara, M, Fujisawa Ogata, TH, Watanabe, N, Asakura, H, Aiso, S, Tsuru, S & Tsuchiya, M 1989, 'Release of the serum immunosuppressive factor by monocytes in patients with crohn's disease', Journal of Clinical and Laboratory Immunology, 巻. 29, 番号 4, pp. 161-166.
Watanabe M, Hibi T, Iwao Y, Kanai T, Ohara M, Fujisawa Ogata TH その他. Release of the serum immunosuppressive factor by monocytes in patients with crohn's disease. Journal of Clinical and Laboratory Immunology. 1989 12 1;29(4):161-166.
Watanabe, M. ; Hibi, T. ; Iwao, Y. ; Kanai, T. ; Ohara, M. ; Fujisawa Ogata, T. H. ; Watanabe, N. ; Asakura, H. ; Aiso, Sadakazu ; Tsuru, S. ; Tsuchiya, M. / Release of the serum immunosuppressive factor by monocytes in patients with crohn's disease. :: Journal of Clinical and Laboratory Immunology. 1989 ; 巻 29, 番号 4. pp. 161-166.
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AU - Hibi, T.

AU - Iwao, Y.

AU - Kanai, T.

AU - Ohara, M.

AU - Fujisawa Ogata, T. H.

AU - Watanabe, N.

AU - Asakura, H.

AU - Aiso, Sadakazu

AU - Tsuru, S.

AU - Tsuchiya, M.

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N2 - We investigated the in vitro immunosuppressive effect of the sera and the culture supernatants of the peripheral blood mononuclear cells obtained from patients with Crohn's disease. Sera from Crohn's disease markedly suppressed the proliferative response of mouse spleen cells, compared with sera from ulcerative colitis, intestinal tuberculosis and normal controls. The culture supernatants of the adherent mononuclear cells from Crohn's disease showed a remarkable suppressive effect (96±1%), while the culture supernatants of the nonadherent mononuclear cells ha no suppressive activity (-10±16%). The culture supernatants of the adherent and non-adherent mononuclear cells from normal controls had no suppressive activity. The fractionization of the culture supernatants of the adherent cells from the Crohn's disease patients demonstrated that the fractions with high suppressive activity had similar or identical biochemical properties to the serum immunosuppressive fractions which has been reported previously. These results indicate that monocytes may release the serum immunosuppressive factor in Crohn's disease. This factor may contribute to the depression of the immune reactivity in the mucosal lesion and to the persistence of the stimulation of abnormal immune response in the intestinal wall.

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