抄録
Techniques for patterned modification of substrate sur faces are important for forming microarrays on protein chips. A hexam-ethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and the resulting surface was partially modified by sub sequent nitrogen plasma treatment with a patterned shadow mask. When surface adsorption of an antibody protein (F(ab')2 fragment of anti-human immunoglobulin G) was visualized by fluorescence microscopy, distinct 80 x 80 μm2 square spots were observed, surrounded by a non-fluorescent 80 μmi-wide grid. This pattern could be attributed to proteins selectively ad sorbed onto the nitrogen plasma-treated regions but not onto the surface of pristine HMDS PPF. This provided a simple fabrication method of protein patterning.
| 元の言語 | English |
|---|---|
| ページ（範囲） | 978-980 |
| ページ数 | 3 |
| ジャーナル | IEICE Transactions on Electronics |
| 巻 | E91-C |
| 発行部数 | 6 |
| DOI | |
| 出版物ステータス | Published - 2008 6 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Electronic, Optical and Magnetic Materials
これを引用
Selective adsorption of an antibody onto a plasma-polymerized film for protein patterning. / Hiratsuka, Atsunori; Murata, Naoya; Muguruma, Hitoshi; Matsumura, Kazunari.
：: IEICE Transactions on Electronics, 巻 E91-C, 番号 6, 06.2008, p. 978-980.研究成果: Article
}
TY - JOUR
T1 - Selective adsorption of an antibody onto a plasma-polymerized film for protein patterning
AU - Hiratsuka, Atsunori
AU - Murata, Naoya
AU - Muguruma, Hitoshi
AU - Matsumura, Kazunari
PY - 2008/6
Y1 - 2008/6
N2 - Techniques for patterned modification of substrate sur faces are important for forming microarrays on protein chips. A hexam-ethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and the resulting surface was partially modified by sub sequent nitrogen plasma treatment with a patterned shadow mask. When surface adsorption of an antibody protein (F(ab')2 fragment of anti-human immunoglobulin G) was visualized by fluorescence microscopy, distinct 80 x 80 μm2 square spots were observed, surrounded by a non-fluorescent 80 μmi-wide grid. This pattern could be attributed to proteins selectively ad sorbed onto the nitrogen plasma-treated regions but not onto the surface of pristine HMDS PPF. This provided a simple fabrication method of protein patterning.
AB - Techniques for patterned modification of substrate sur faces are important for forming microarrays on protein chips. A hexam-ethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and the resulting surface was partially modified by sub sequent nitrogen plasma treatment with a patterned shadow mask. When surface adsorption of an antibody protein (F(ab')2 fragment of anti-human immunoglobulin G) was visualized by fluorescence microscopy, distinct 80 x 80 μm2 square spots were observed, surrounded by a non-fluorescent 80 μmi-wide grid. This pattern could be attributed to proteins selectively ad sorbed onto the nitrogen plasma-treated regions but not onto the surface of pristine HMDS PPF. This provided a simple fabrication method of protein patterning.
KW - Adsorp tion
KW - Antibody
KW - F(ab') fragment
KW - Patterning
KW - Plasma-polymerized film
UR - http://www.scopus.com/inward/record.url?scp=77049120035&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77049120035&partnerID=8YFLogxK
U2 - 10.1093/ietele/e91-c.6.978
DO - 10.1093/ietele/e91-c.6.978
M3 - Article
AN - SCOPUS:77049120035
VL - E91-C
SP - 978
EP - 980
JO - IEICE Transactions on Electronics
JF - IEICE Transactions on Electronics
SN - 0916-8524
IS - 6
ER -