Selective adsorption of an antibody onto a plasma-polymerized film for protein patterning

Atsunori Hiratsuka, Naoya Murata, Hitoshi Muguruma, Kazunari Matsumura

研究成果: Article

5 引用 (Scopus)

抜粋

Techniques for patterned modification of substrate sur faces are important for forming microarrays on protein chips. A hexam-ethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and the resulting surface was partially modified by sub sequent nitrogen plasma treatment with a patterned shadow mask. When surface adsorption of an antibody protein (F(ab')2 fragment of anti-human immunoglobulin G) was visualized by fluorescence microscopy, distinct 80 x 80 μm2 square spots were observed, surrounded by a non-fluorescent 80 μmi-wide grid. This pattern could be attributed to proteins selectively ad sorbed onto the nitrogen plasma-treated regions but not onto the surface of pristine HMDS PPF. This provided a simple fabrication method of protein patterning.

元の言語English
ページ(範囲)978-980
ページ数3
ジャーナルIEICE Transactions on Electronics
E91-C
発行部数6
DOI
出版物ステータスPublished - 2008 6

    フィンガープリント

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

これを引用