Small volume low mechanical stress cytometry using computer-controlled Braille display microfluidics

Yi Chung Tung, Yu Suke Torisawa, Nobuyuki Futai, Shuichi Takayama

研究成果: Article

31 引用 (Scopus)

抄録

This paper describes a micro flow cytometer system designed for efficient and non-damaging analysis of samples with small numbers of precious cells. The system utilizes actuation of Braille-display pins for micro-scale fluid manipulation and a fluorescence microscope with a CCD camera for optical detection. The microfluidic chip is fully disposable and is composed of a polydimethylsiloxane (PDMS) slab with microchannel features sealed against a thin deformable PDMS membrane. The channels are designed with diffusers to alleviate pulsatile flow behaviors inherent in pin actuator-based peristaltic pumping schemes to maximize hydrodynamic focusing of samples with minimal disturbances in the laminar streams within the channel. A funnel connected to the microfluidic channel is designed for efficient loading of samples with small number of cells and is also positioned on the chip to prevent physical damages of the samples by the squeezing actions of Braille pins during actuation. The sample loading scheme was characterized by both computational fluidic dynamics (CFD) simulation and experimental observation. A fluorescein solution was first used for flow field investigation, followed by use of fluorescence beads with known relative intensities for optical detection performance calibration. Murine myoblast cells (C2C12) were exploited to investigate cell viability for the sample loading scheme of the device. Furthermore, human promyelocytic leukemia (HL60) cells stained by hypotonic DNA staining buffer were also tested in the system for cell cycle analysis. The ability to efficiently analyze cellular samples where the number of cells is small was demonstrated by analyzing cells from a single embryoid body derived from mouse embryonic stem cells. Consequently, the designed microfluidic device reported in this paper is promising for easy-to-use, small sample size flow cytometric analysis, and has potential to be further integrated with other Braille display-based microfluidic devices to facilitate a multi-functional lab-on-a-chip for mammalian cell manipulations.

元の言語English
ページ(範囲)1497-1503
ページ数7
ジャーナルLab on a Chip - Miniaturisation for Chemistry and Biology
7
発行部数11
DOI
出版物ステータスPublished - 2007
外部発表Yes

Fingerprint

Microfluidics
Display devices
Cells
Polydimethylsiloxane
Fluorescence
Pulsatile flow
Lab-on-a-chip
Fluidics
CCD cameras
Microchannels
Stem cells
Fluorescein
Flow fields
Buffers
Microscopes
DNA
Actuators
Hydrodynamics
Calibration
Membranes

ASJC Scopus subject areas

  • Clinical Biochemistry

これを引用

Small volume low mechanical stress cytometry using computer-controlled Braille display microfluidics. / Tung, Yi Chung; Torisawa, Yu Suke; Futai, Nobuyuki; Takayama, Shuichi.

:: Lab on a Chip - Miniaturisation for Chemistry and Biology, 巻 7, 番号 11, 2007, p. 1497-1503.

研究成果: Article

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