抄録
The organ-specific and temporal control of gene activation/inactivation is a key issue in the understanding of protein function during normal and pathological development and during oncogenesis. We generated transgenic mice bearing a tamoxifen-dependent Cre recombinase (Tyr::Cre-ERT2) gene expressed under the control of a 6.1 kb murine tyrosinase promoter in order to facilitate targeted spatiotemporally controlled somatic recombination in melanoblasts/melanocytes. Cre-ERT2 production was detected in tissues containing melanocytes. After tamoxifen induction at various times during embryogenesis and adulthood in a Cre-responsive reporter mouse strain, genetic recombination was detected in the melanoblasts and melanocytes of the skin. Thus, the Tyr::Cre-ERT2 transgenic mice provides a valuable tool for following this cell lineage and for investigating gene function in melanocyte development and transformation.
本文言語 | English |
---|---|
ページ(範囲) | 34-43 |
ページ数 | 10 |
ジャーナル | Genesis |
巻 | 44 |
号 | 1 |
DOI | |
出版ステータス | Published - 2006 1月 1 |
外部発表 | はい |
ASJC Scopus subject areas
- 遺伝学
- 内分泌学
- 細胞生物学