Structure of ige epitopes on a new 39-KD allergen molecule from the house dust mite, dermatophagoides farina

Tsunehiro Aki, Kazuhisa Ono, Yuuji Hidaka, Yasutsugu Shimonishi, Toshihiko Jyo, Takeshi Wada, Mitsuo Yamashita, Seiko Shigeta, Yoshikatsu Murooka, Satoru Oka

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Two IgE epitope sequences comprising Ser56-Pro-Val-Thr-Lys-Arg-Ala-Ser- Leu-Lys-Ile-Asp-Ser-Lys-Lys70 and Asp104-Val-GIu-Leu-Ser-Leu-Arg-Ser-Ser-Asp-Ile-Ala115 were identified by deletion analysis of the cDNA encoding a new 39-kD protein of mite allergen. A syntheticdodecapeptide corresponding to the latter epitope sequence functioned as a monovalent and mite-specific hapten. Replacement of each of the 12 amino acid residues with Gly, using site-directed mutagenesis, indicated that Arg110 may play a central role in IgE binding. However, the 8 allergic sera tested exhibited a wide variation in their amino acid residues required for reactivity to IgE in allergic sera.

本文言語English
ページ(範囲)357-364
ページ数8
ジャーナルInternational Archives of Allergy and Immunology
103
4
DOI
出版ステータスPublished - 1994
外部発表はい

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

フィンガープリント 「Structure of ige epitopes on a new 39-KD allergen molecule from the house dust mite, dermatophagoides farina」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル