Structures of (-)-epicatechin glucuronide identified from plasma and urine after oral ingestion of (-)-epicatechin: Differences between human and rat

Midori Natsume, Naomi Osakabe, Makoto Oyama, Motoko Sasaki, Seigo Baba, Yoshimasa Nakamura, Toshihiko Osawa, Junji Terao

研究成果: Article

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(-)-Epicatechin is one of the most potent antioxidants present in the human diet. Particularly high levels are found in black tea, apples, and chocolate. High intake of catechins has been associated with reduced risk of cardiovascular diseases. There have been several reports concerning the bioavailability of catechins, however, the chemical structure of (-)-epicatechin metabolites in blood, tissues, and urine remains unclear. In the present study, we purified and elucidated the chemical structure of (-)-epicatechin metabolites in human and rat urine after oral administration. Three metabolites were purified from human urine including (-)-epicatechin-3′-O-glucuronide, 4′-O-methyl-(-)-epicatechin-3′-O-glucuronide, and 4′-O-methyl-(-)-epicatechin-5 or 7-O-glucuronide, according to 1H- and 13C-NMR, HMBC, and LC-MS analyses. The metabolites purified from rat urine were 3′-O-methyl-(-)-epicatechin, (-)-epicatechin-7-O-glucuronide, and 3′-O-methyl-(-)-epicatechin-7-O-glucuronide. These compounds were also detected in the blood of humans and rats by LC-MS. The presence of these metabolites in blood and urine suggests that catechins are metabolized and circulated in the body after administration of catechin-containing foods.

元の言語English
ページ(範囲)840-849
ページ数10
ジャーナルFree Radical Biology and Medicine
34
発行部数7
DOI
出版物ステータスPublished - 2003 4 1
外部発表Yes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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