@article{0fa2df2c5d8a4804a6f3b7142ff70a2c,
title = "Synthesis and development of biologically active fluorescent-labeled vitamin K analogues and monitoring of their subcellular distribution",
abstract = "New fluorescent analogues of menaquinone-4 and phylloquinone were prepared and their subcellular distribution monitored using a confocal laser scanning microscope. These analogues incorporate an FITC group anchored to the naphthoquinone skeleton through an amide bond expected to be resistant to metabolism. On their addition to the culture medium, fluorescence was readily observed inside a human osteosarcoma cell line. This result indicates that the fluorescent analogues penetrate into cells the same as vitamin K, and therefore, would be useful for achieving insight into the action mechanism of vitamin K.",
keywords = "Fluorescent-label, Menaquinone-4, Phylloquinone, Subcellular distribution, Vitamin K",
author = "Yoshitomo Suhara and Shinya Abe and Aya Murakami and Yuka Shimomura and Kimie Nakagawa and Maya Kamao and Naoko Tsugawa and Toshio Okano",
note = "Funding Information: We are grateful to Dr. Makiko Sugiura, Dr. Atsuko Takeuchi, and Dr. Chisato Tode for the spectroscopic measurements. We also thank Eisai Co., Ltd for generously providing the intermediate of the vitamin K analogues. This study was supported in part by a Grant-in-Aid for Scientific Research (C) (grant number 18590112) from the Japan Society for the Promotion of Science. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",
year = "2008",
month = sep,
day = "8",
doi = "10.1016/j.tet.2008.06.085",
language = "English",
volume = "64",
pages = "8789--8796",
journal = "Tetrahedron",
issn = "0040-4020",
publisher = "Elsevier Limited",
number = "37",
}