Synthesis and development of biologically active fluorescent-labeled vitamin K analogues and monitoring of their subcellular distribution

Yoshitomo Suhara, Shinya Abe, Aya Murakami, Yuka Shimomura, Kimie Nakagawa, Maya Kamao, Naoko Tsugawa, Toshio Okano

研究成果: Article査読

4 被引用数 (Scopus)

抄録

New fluorescent analogues of menaquinone-4 and phylloquinone were prepared and their subcellular distribution monitored using a confocal laser scanning microscope. These analogues incorporate an FITC group anchored to the naphthoquinone skeleton through an amide bond expected to be resistant to metabolism. On their addition to the culture medium, fluorescence was readily observed inside a human osteosarcoma cell line. This result indicates that the fluorescent analogues penetrate into cells the same as vitamin K, and therefore, would be useful for achieving insight into the action mechanism of vitamin K.

本文言語English
ページ(範囲)8789-8796
ページ数8
ジャーナルTetrahedron
64
37
DOI
出版ステータスPublished - 2008 9月 8
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 創薬
  • 有機化学

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