The prolactin gene is expressed in the mouse kidney

Yukinao Sakai, Yoshiki Hiraoka, Motoyuki Ogawa, Yuji Takeuchi, Sadakazu Aiso

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Background. Prolactin (PRL), originally identified as an anterior pituitary hormone exhibiting lactogenic activity, is now recognized as a versatile hormone expressed in a wide variety of tissues. Methods. In this study, the expression of PRL in the mouse kidney was investigated by solution-phase and in situ reverse transcription-polymerase chain reaction (RT-PCR) methods and immunohistochemistry. Results. Mouse PRL (mPRL) transcript and protein are localized in the parietal epithelial cells of Bowman's capsule. Pit-1 is a positive transcription factor for the expression of the PRL gene. The presence of Pit-1 transcript in the kidney was also assessed by RT-PCR methods. The localization of Pit-1 mRNA coincided well with that of PRL. Immunoreactivity to mouse PRL receptor (mPRL-R) is distributed on the luminal membrane of the proximal tubule cells and the parietal epithelial cells of Bowman's capsule. Conclusion. These data indicate that the parietal epithelial cells of Bowman's capsule synthesize PRL de novo and suggest that Pit-1 contributes to the transcriptional regulation of PRL gene expression in the kidney, and PRL expressed in this tissue functions in an autocrine/paracrine fashion.

本文言語English
ページ(範囲)833-840
ページ数8
ジャーナルKidney International
55
3
DOI
出版ステータスPublished - 1999
外部発表はい

ASJC Scopus subject areas

  • 腎臓病学

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