Characterization of grafted vascular scaffold is essential for evaluating its biocompatibility, maturity and structural intensity. Quantitative elastic modulus measurement of vascular scaffold powerfully assists its characterization because the mechanical property is closely related with the biocompatibility, maturity and structural intensity of the vascular scaffold. We have presented an in vitro or in vivo measurement system for the tissue-engineered vessel before and after grafting in the previous study, we present a novel and modified method for in vivo assessment and follow-up of the vascular scaffold in this study. The measured quantitative elastic modulus is converted into the regeneration score for in vivo assessment. The effectiveness of the proposed evaluation method was validated by in vivo experimental data using canines.