Visualization of dystrophic muscle fibers in mdx mouse by vital staining with evans blue: Evidence of apoptosis in dystrophin-deficient muscle

Ryoichi Matsuda, Akio Nishikawa, Hideho Tanaka

研究成果: Article査読

272 被引用数 (Scopus)

抄録

Degenerating muscle fibers in the skeletal muscle of mdx mice were visualized by vital staining with Evans blue. Evans blue injected intravenously stained only degenerating muscle fibers which were visible as blue fibers macroscopically and could also be seen as red fluorescent fibers microscopically. Evans blue-stained muscle fibers were either hypercontracted or degrading. Intact or regenerating muscle fibers in mdx mice and muscle fibers in B10 control mice were not stained with the dye. DNA isolated from Evans blue-stained fibers exhibited fragmentation to approximately 180 base pairs on agarose gel electrophoresis. Such DNA fragmentation was not found in DNA from unstained muscle fibers in mdx or B10 mice. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL)-positive myonuclei were also found in Evans blue-stained muscle fibers but not in unstained ones. These results indicate that degenerating muscle fibers in the mdx mouse show an increase in membrane permeability and undergo apoptosis. Vital staining with Evans blue is useful not only for distinguishing degenerating muscle fibers, but also for studying the degeneration process biochemically in dystrophin-deficient muscle. This method may also be useful for evaluating the therapeutic effects of drug administration, gene transfer, and myoblast transfer in the mdx mouse.

本文言語English
ページ(範囲)959-963
ページ数5
ジャーナルJournal of biochemistry
118
5
DOI
出版ステータスPublished - 1995 10
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学

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