Vitamin E deficiency induces axonal degeneration in mouse hippocampal neurons

Koji Fukui, Hiroaki Kawakami, Tatsuki Honjo, Reiko Ogasawara, Hirokatsu Takatsu, Tadashi Shinkai, Tatsuro Koike, Shiro Urano

研究成果: Article

16 引用 (Scopus)

抄録

Several lines of evidence demonstrate the relationship between vitamin E deficiency and cognitive dysfunction in rodent models, but little is known about the underlying mechanisms. In this study, we found axonal injury in the hippocampal CA1 region of vitamin E-deficient and normal old mice using immunohistochemical assay. The number of cells in the hippocampal CA1 region of vitamin E-deficient mice and normal old mice was significantly lower than in normal young mice. It is well known that collapsin response mediator protein (CRMP)-2 plays a crucial role in the maintenance of axonal conditions. The expressions of CRMP-2 in the cerebral cortex and hippocampus of vitamin E-deficient mice were significantly lower than both the regions of normal ones. In normal old mice, the expression of CRMP-2 in the cerebral cortex was significantly lower than in the normal ones. In addition, the appearance of microtubule-associated protein (MAP)-light chain 3 (LC3), a major index of autophagy, was higher in the cerebral cortex and hippocampus of vitamin E-deficient mice than in normal young and old mice. These results indicate that axonal degeneration is induced in living tissues, but not cultured cells, and that changes in CRMP-2 and MAP-LC3 may underlie vitamin E-deficiency-related axonal degeneration.

元の言語English
ページ(範囲)377-383
ページ数7
ジャーナルJournal of Nutritional Science and Vitaminology
58
発行部数6
DOI
出版物ステータスPublished - 2012

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

これを引用

Vitamin E deficiency induces axonal degeneration in mouse hippocampal neurons. / Fukui, Koji; Kawakami, Hiroaki; Honjo, Tatsuki; Ogasawara, Reiko; Takatsu, Hirokatsu; Shinkai, Tadashi; Koike, Tatsuro; Urano, Shiro.

:: Journal of Nutritional Science and Vitaminology, 巻 58, 番号 6, 2012, p. 377-383.

研究成果: Article

Fukui, K, Kawakami, H, Honjo, T, Ogasawara, R, Takatsu, H, Shinkai, T, Koike, T & Urano, S 2012, 'Vitamin E deficiency induces axonal degeneration in mouse hippocampal neurons', Journal of Nutritional Science and Vitaminology, 巻. 58, 番号 6, pp. 377-383. https://doi.org/10.3177/jnsv.58.377
Fukui, Koji ; Kawakami, Hiroaki ; Honjo, Tatsuki ; Ogasawara, Reiko ; Takatsu, Hirokatsu ; Shinkai, Tadashi ; Koike, Tatsuro ; Urano, Shiro. / Vitamin E deficiency induces axonal degeneration in mouse hippocampal neurons. :: Journal of Nutritional Science and Vitaminology. 2012 ; 巻 58, 番号 6. pp. 377-383.
@article{bb38393b2ef14db29d76b367d922225f,
title = "Vitamin E deficiency induces axonal degeneration in mouse hippocampal neurons",
abstract = "Several lines of evidence demonstrate the relationship between vitamin E deficiency and cognitive dysfunction in rodent models, but little is known about the underlying mechanisms. In this study, we found axonal injury in the hippocampal CA1 region of vitamin E-deficient and normal old mice using immunohistochemical assay. The number of cells in the hippocampal CA1 region of vitamin E-deficient mice and normal old mice was significantly lower than in normal young mice. It is well known that collapsin response mediator protein (CRMP)-2 plays a crucial role in the maintenance of axonal conditions. The expressions of CRMP-2 in the cerebral cortex and hippocampus of vitamin E-deficient mice were significantly lower than both the regions of normal ones. In normal old mice, the expression of CRMP-2 in the cerebral cortex was significantly lower than in the normal ones. In addition, the appearance of microtubule-associated protein (MAP)-light chain 3 (LC3), a major index of autophagy, was higher in the cerebral cortex and hippocampus of vitamin E-deficient mice than in normal young and old mice. These results indicate that axonal degeneration is induced in living tissues, but not cultured cells, and that changes in CRMP-2 and MAP-LC3 may underlie vitamin E-deficiency-related axonal degeneration.",
keywords = "Aging, Autophagy, Axon, CRMP-2, Vitamin E",
author = "Koji Fukui and Hiroaki Kawakami and Tatsuki Honjo and Reiko Ogasawara and Hirokatsu Takatsu and Tadashi Shinkai and Tatsuro Koike and Shiro Urano",
year = "2012",
doi = "10.3177/jnsv.58.377",
language = "English",
volume = "58",
pages = "377--383",
journal = "Journal of Nutritional Science and Vitaminology",
issn = "0301-4800",
publisher = "Center for Academic Publications Japan",
number = "6",

}

TY - JOUR

T1 - Vitamin E deficiency induces axonal degeneration in mouse hippocampal neurons

AU - Fukui, Koji

AU - Kawakami, Hiroaki

AU - Honjo, Tatsuki

AU - Ogasawara, Reiko

AU - Takatsu, Hirokatsu

AU - Shinkai, Tadashi

AU - Koike, Tatsuro

AU - Urano, Shiro

PY - 2012

Y1 - 2012

N2 - Several lines of evidence demonstrate the relationship between vitamin E deficiency and cognitive dysfunction in rodent models, but little is known about the underlying mechanisms. In this study, we found axonal injury in the hippocampal CA1 region of vitamin E-deficient and normal old mice using immunohistochemical assay. The number of cells in the hippocampal CA1 region of vitamin E-deficient mice and normal old mice was significantly lower than in normal young mice. It is well known that collapsin response mediator protein (CRMP)-2 plays a crucial role in the maintenance of axonal conditions. The expressions of CRMP-2 in the cerebral cortex and hippocampus of vitamin E-deficient mice were significantly lower than both the regions of normal ones. In normal old mice, the expression of CRMP-2 in the cerebral cortex was significantly lower than in the normal ones. In addition, the appearance of microtubule-associated protein (MAP)-light chain 3 (LC3), a major index of autophagy, was higher in the cerebral cortex and hippocampus of vitamin E-deficient mice than in normal young and old mice. These results indicate that axonal degeneration is induced in living tissues, but not cultured cells, and that changes in CRMP-2 and MAP-LC3 may underlie vitamin E-deficiency-related axonal degeneration.

AB - Several lines of evidence demonstrate the relationship between vitamin E deficiency and cognitive dysfunction in rodent models, but little is known about the underlying mechanisms. In this study, we found axonal injury in the hippocampal CA1 region of vitamin E-deficient and normal old mice using immunohistochemical assay. The number of cells in the hippocampal CA1 region of vitamin E-deficient mice and normal old mice was significantly lower than in normal young mice. It is well known that collapsin response mediator protein (CRMP)-2 plays a crucial role in the maintenance of axonal conditions. The expressions of CRMP-2 in the cerebral cortex and hippocampus of vitamin E-deficient mice were significantly lower than both the regions of normal ones. In normal old mice, the expression of CRMP-2 in the cerebral cortex was significantly lower than in the normal ones. In addition, the appearance of microtubule-associated protein (MAP)-light chain 3 (LC3), a major index of autophagy, was higher in the cerebral cortex and hippocampus of vitamin E-deficient mice than in normal young and old mice. These results indicate that axonal degeneration is induced in living tissues, but not cultured cells, and that changes in CRMP-2 and MAP-LC3 may underlie vitamin E-deficiency-related axonal degeneration.

KW - Aging

KW - Autophagy

KW - Axon

KW - CRMP-2

KW - Vitamin E

UR - http://www.scopus.com/inward/record.url?scp=84874183380&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84874183380&partnerID=8YFLogxK

U2 - 10.3177/jnsv.58.377

DO - 10.3177/jnsv.58.377

M3 - Article

C2 - 23419395

AN - SCOPUS:84874183380

VL - 58

SP - 377

EP - 383

JO - Journal of Nutritional Science and Vitaminology

JF - Journal of Nutritional Science and Vitaminology

SN - 0301-4800

IS - 6

ER -