Wnt5a promotes adhesion of human dermal fibroblasts by triggering a phosphatidylinositol-3 kinase/Akt signal

Aya Kawasaki, Kosuke Torii, Yuki Yamashita, Koji Nishizawa, Kohsuke Kanekura, Masanori Katada, Minoru Ito, Ikuo Nishimoto, Kenzo Terashita, Sadakazu Aiso, Masaaki Matsuoka

研究成果: Article査読

43 被引用数 (Scopus)

抄録

Frizzled-3 (Fzd3), highly expressed in both the central nervous system (CNS) and skin, plays essential roles in axonal growth and guidance during the CNS development and may be involved in maintenance of skin integrity, although its ligand remains undetermined. In this study, we demonstrate that Wnt5a specifically binds to Fzd3 in vitro and triggers phosphorylation of Akt mediated by phosphatidylinositol-3 kinase (PI3K), but not that of ERK or protein kinase C, in human primary-cultured dermal fibroblasts. We have further found that such Wnt5a/Fzd3-triggered activation of the PI3K/Akt signal promotes integrin-mediated adhesion of human dermal fibroblasts to collagen I-coated dishes. Based on another finding that Wnt5a/Fzd3-triggered activation of the PI3K/Akt signal was blocked by an excess amount of a recombinant Fzd3-cysteine-rich domain (CRD), but not by that of a recombinant Fzd6-CRD, it is concluded that Wnt5a is a natural ligand of Fzd3 that triggers the PI3K/Akt signal and promotes adhesion of human dermal fibroblasts.

本文言語English
ページ(範囲)2498-2506
ページ数9
ジャーナルCellular Signalling
19
12
DOI
出版ステータスPublished - 2007 12 1
外部発表はい

ASJC Scopus subject areas

  • 細胞生物学

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